Identification of Differentially Expressed Genes During Embryogenesis in Maritime Pine (Pinus pinaster)
Differential display of Poly (A+) RNA isolated from zygotic embryos of maritime pine (Pinus pinaster) in 9 stages of development was used to isolate several cDNAs of differentially expressed genes during embryo development. Many cDNA sequences share low similarities to any known DNA or protein sequences but 5 transcripts are predicted to encode homologues of DNA-binding proteins, GTP-binding proteins, lipid transferases and dehydrogenases. A full-length cDNA clone was isolated for a gene (named PpAAI-LTSS 1) whose expression appears to be specific to middle stages of development. The full-length cDNA was also isolated for two clones (named PpRab1 and PpCR1) specific to an early stage of embryo development.
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Digital revista |
| Language: | English |
| Published: |
Unidade de Silvicultura e Produtos Florestais
2005
|
| Online Access: | http://scielo.pt/scielo.php?script=sci_arttext&pid=S0870-63522005000200005 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|