Stenotrophomonas maltophilia a new biocontrol agent against the cowpea weevil Callosobruchus maculatus F. (Coleoptera: Bruchidae)

ABSTRACT Callosobruchus maculatus F. (Coleoptera; Bruchidae) is one of the most important storage pests, especially in legumes, which causes significant damage in many parts of Iran, including east Azarbaijan province. This study aimed to isolate and identify the insect-associated bacteria with this pest and then evaluate the capability of these bacterial isolates in the biocontrol of this insect in laboratory conditions. Stock culture of C. maculatus was collected from the field in East Azerbaijan, and then was maintained under laboratory conditions of 25 ± 2° C and 60 ± 5% RH. Dead adult beetles were surface-sterilized with 0.1% hypochlorite and then transferred to NA media plates. It appeared that bacterial isolates were isolated and purified. In other to perform a bioassay, a bacterial suspension with a concentration of 106 (cell/ml) was prepared from each bacterial isolates were then spread in three replications on the same-age pest population separately. After the recording of the pest mortality, the most effective bacterial isolate, which showed over 40% mortality, was selected for supplementary assays selected bacterial isolate was identified by PCR using universal 16s rDNA primers. The obtained sequence data from our experiment were compared with gene bank sequences, and phylogenetic tree was drown. Based on sequencing data, the isolate bacteria are identified as Stenotrophomonas maltophilia species. The supplementary biochemical tests were confirmed the molecular data. These data is the first report for the biocontrol capability of this bacteria on this insect.

Bibliographic Details

Main Authors:	Soleimani,Hosein, Jamshidi,Manizheh, Khakvar,Reza, Masoomeh Hasheminia,Seyedeh
Format:	Digital revista
Language:	English
Published:	Universidad de Tarapacá. Facultad de Ciencias Agronómicas 2022
Online Access:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-34292022000400017
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