VALIDATION OF A METHOD TO DETECT CENTRAL NERVOUS SYSTEM ACTING DRUGS IN BLOOD BY GC/MS. APPLICATION IN CASES OF DEATH CAUSED BY PHENOTHIAZINES IN THE NORTH OF CHILE

A simple and rapid analytical method is developed and validated for the determination of antiepileptic and antipsychotic drugs in human whole blood using a solid-phase extraction and quantification by gas chromatography-mass spectrometry (GC/MS). Prazepam was used as internal standard (IS). The specificity, linearity, intra- and inter-assay precision and accuracy, and extraction recovery were fully evaluated. The limits of detection and quantification were in the range of 0.52 - 0.98 &#956;g/mL and 1.56 - 2.97 &#956;g/mL for antiepileptic drugs (pentobarbital, phenobarbital and carbamazepine) and 1.31 - 2.94 ng/mL and 3.98 - 8.89 ng/mL for antipsychotic drugs (chlorpromazine and thioridazine), respectively. The relative standard deviation (RSD) was less than 7.07%, while the intra-day accuracy was < 3.21% and the inter-day was < 8.80%, referred to RSD. The developed methodology was applied in forensic cases of death caused by phenothiazines in the North of Chile during 2010-2012.

Bibliographic Details

Main Authors:	BRAVO,FELIPE, ULLOA,ROBERTO, VENEGAS,KARINA, CONTZEN,MARIA CECILIA, RIOS,DAVID, BRICEÑO,MARGARITA, CALDERONI,PEDRO BUC, BENITES,JULIO
Format:	Digital revista
Language:	English
Published:	Sociedad Chilena de Química 2014
Online Access:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072014000300016
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