Ethanol mediated enhancement in bacterial transformation
In molecular biology, transformation using E. coli as a host plays a key role in synthesizing gene libraries. The present study demonstrated a new ethanol-based method for transformation of plasmid DNA to E. coli. Ethanol at 10% concentration (v/v) showed best results. Further, as compared with traditional CaCl2 method, the transformation rate, using protocol outlined in this study, was very high, suggesting amenable for further applications.
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| Main Authors: | , , |
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| Format: | Digital revista |
| Language: | English |
| Published: |
Pontificia Universidad Católica de Valparaíso
2007
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| Online Access: | http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000100016 |
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