Qualitative and quantitative detection of SARS-CoV-2 RNA in untreated wastewater in Western Cape Province, South Africa

Recent studies have shown that the detection of SARS-CoV-2 genetic material in wastewater may provide the basis for a surveillance system to track the environmental dissemination of this virus in communities. An effective wastewater-based epidemiology (WBE) system may prove critical in South Africa (SA), where health systems infrastructure, testing capacity, personal protective equipment and human resource capacity are constrained. In this proof-of-concept study, we investigated the potential of SARS-CoV-2 RNA surveillance in untreated wastewater as the basis for a system to monitor COVID-19 prevalence in the population, an early warning system for increased transmission, and a monitoring system to assess the effectiveness of interventions. The laboratory confirmed the presence (qualitative analysis) and determined the RNA copy number of SARS-CoV-2 viral RNA by reverse transcription polymerase chain reaction (quantitative) analysis from 24-hour composite samples collected on 18 June 2020 from five wastewater treatment plants in Western Cape Province, SA. The study has shown that a WBE system for monitoring the status and trends of COVID-19 mass infection in SA is viable, and its development and implementation may facilitate the rapid identification of hotspots for evidence-informed interventions.

Bibliographic Details

Main Authors:	Johnson,R, Muller,C J F, Ghoor,S, Louw,J, Archer,E, Surujlal-Naicker,S, Berkowitz,N, Volschenk,M, Brocker,L H L, Wolfaardt,G, van der Walt,M, Mutshembele,A M, Malema,S, Gelderblom,H C, Mdhluli,M, Gray,G, Mathee,A, Street,R A
Format:	Digital revista
Language:	English
Published:	South African Medical Association 2021
Online Access:	http://www.scielo.org.za/scielo.php?script=sci_arttext&pid=S0256-95742021000300011
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