Bacterial Cellulose Production by Komagataeibacter hansenii ATCC 23769 Using Sisal Juice - An Agroindustry Waste
ABSTRACT In this study, we have optimized production of bacterial cellulose (BC) by Komagataeibacter hansenii ATCC 23769 in a static cultivation using sisal juice, an agroindustrial residue, as substrate. Optimization of fermentation parameters has been carried out using the one-variable-at-a-time method. Effect of initial sugar concentration, pH, nitrogen supplement, and cultivation time was evaluated. The influence of nitrogen source and quantity for bacterial cellulose production was studied using a central composite rotational design (CCRD).The highest production of BC (3.38 g/L) was obtained after 10 days of cultivation, using sisal juice (pH 5) at 15 g/L of sugars and supplemented with 7.5 g/L of extract yeast. The cellulose production yield in selected sisal culture conditions was three times higher than the yield in synthetic medium, indicating that sisal juice is a suitable substrate for BC production.
| Main Authors: | , , , , , , , , , |
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| Format: | Digital revista |
| Language: | English |
| Published: |
Brazilian Society of Chemical Engineering
2017
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| Online Access: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-66322017000300671 |
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