A pseudobiospecific hollow fiber cartridge for in vitro adsorption of autoantibodies from pathological serum
The affinity filtration technique using histidine as a pseudobiospecific ligand immobilized on poly(ethylene vinyl alcohol) hollow fiber membranes (His-PEVA) was used to remove autoantibodies from serum of patients with autoimmune disease. The effects of buffering solution conditions on the efficiency of autoantibodies removal was studied. The removal of anti-dsDNA, anti-SS-A/Ro, anti-Sm, anti-Sm/RNP and anti-cardiolipin autoantibodies present in the serum was investigated, comparing the efficiency between Hepes and Tris-HCl buffers. The results showed the potential of the membrane to remove all the autoantibodies studied. Anti SS-A/Ro was removed more efficiently in Tris-HCl buffer system rather than with the Hepes buffer.
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Digital revista |
| Language: | English |
| Published: |
Brazilian Society of Chemical Engineering
2000
|
| Online Access: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-66322000000400052 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|