Contribution of environmental factors in the formation of biofilms by Alicyclobacillus acidoterrestris on surfaces of the orange juice industry

ABSTRACT: The objective of this study was to evaluate the effect of the initial microbial load, temperature and contact time on the biofilm formation of Alicyclobacillus acidoterrestris on stainless steel and natural food-grade rubber using orange juice as culture medium. The low initial load of A. acidoterrestris (2 log CFU/mL) led to biofilm formation on the stainless steel surface after 48 h of contact at 28 ºC and after 24 h at 45 ºC, and on natural food-grade rubber surface after 48 h of contact at both temperatures. The high initial microbial load (5 log CFU/mL) led to biofilm formation on stainless steel after 4 h of contact at 28 °C and 45 °C, while biofilm was formed on natural food-grade rubber after 8 h of contact at 28 °C and 4 h at 45 °C. The microbial load also affected the presence of spores in biofilm, which was observed on both surfaces only at high initial loads of A. acidoterrestris.

Bibliographic Details

Main Authors:	Dutra,Tatiane Viana, Prado,Daniela Biral do, Anjos,Márcia Maria dos, Machinski Junior,Miguel, Mikcha,Jane Martha Graton, Abreu Filho,Benício Alves de
Format:	Digital revista
Language:	English
Published:	Universidade Federal de Santa Maria 2020
Online Access:	http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782020000300753
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