Etiologic diagnosis of bovine infectious abortion by PCR

Infectious abortion is a significant cause of reproductive failure and economic losses in cattle. The goal of this study was to detect nucleic acids of several infectious agents known to cause abortion including Arcanobacterium pyogenes, Bovine Herpesvirus 1, Brucella abortus, Campylobacter fetus subsp. venerealis, Chlamydophila abortus, Leptospira sp., Listeria monocytogenes, Salmonella sp., Mycoplasma bovis, Mycoplasma bovigenitalium, Neospora caninum, and Tritrichomonas foetus. Tissue homogenates from 42 fetuses and paraffin-embedded tissues from 28 fetuses and 14 placentas/endometrium were included in this study. Brucella abortus was detected in 14.2% (12/84) of the samples. Salmonella sp. DNA was amplified from 2 fetuses, and there was one positive for Neospora caninum, and another for Listeria monocytogenes. This PCR-based approach resulted in identification of the etiology in 19% of samples, or 20% if considered fetal tissues only.

Bibliographic Details

Main Authors:	Silva,Teane Milagres Augusto da, Oliveira,Raquel Gonzaga de, Mol,Juliana Pinto da Silva, Xavier,Mariana Noyma, Paixão,Tatiane Alves da, Cortez,Adriana, Heinemann,Marcos Bryan, Richtzenhain,Leonardo José, Lage,Andrey Pereira, Santos,Renato de Lima
Format:	Digital revista
Language:	English
Published:	Universidade Federal de Santa Maria 2009
Online Access:	http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782009000900028
Tags:	Add Tag No Tags, Be the first to tag this record!