Protein and antigen profiles of Leptospira interrogans serovar Hardjo

The protein profile of the outer membrane of Leptospira interrogans serovar Hardjo subtype hardjoprajitno associated with the bovine natural immune response was investigated. The outer membrane proteins were extracted utilizing Triton X114 and precipitated with acetone. The protein sample was then resolved by SDS-PAGE and reacted in western blot against sera from a hyperimmune rabbit and from naturally infected bovines. In silver stained gels, 14 protein bands were observed, among which four proteins, with 22, 29, 47 and 63kDa, appeared as major constituents. Western blot tests with hyperimmune rabbit antiserum detected bands corresponding to proteins with 35; 27; 24; 21; 17 and 14kDa, while 32kDa and 45kDa proteins were the most immunoreactive with sera from naturally infected bovines.

Saved in:
Bibliographic Details
Main Authors: Lafetá,Bárbara Nobre, Castro,Elaine Cristina de, Silva,Nivaldo da
Format: Digital revista
Language:English
Published: Universidade Federal de Santa Maria 2009
Online Access:http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782009000900024
Tags: Add Tag
No Tags, Be the first to tag this record!