Amniotic membrane as an option for treatment of acute Achilles tendon injury in rats

Abstract Purpose: To evaluate the effect of human amniotic membrane (hAM) fragment on inflammatory response, proliferation of fibroblast and organization of collagen fibers in injured tendon. Methods: Sixty rats were divided into 3 groups: C - surgical procedures without tendon lesion and with simulation of hAM application; I - surgical procedures, tendon injury and simulation of hAM application; T - surgical procedures, tendon injury and hAM application. These groups were subdivided into four experimental times (3, 7, 14 and 28 days). The samples underwent histological analysis and ATR-FTIR spectroscopy. Results: Histological analysis at 14 days, the T group showed collagen fibers with better alignment. At 28 days, the I group presented the characteristics described for the T group at 14 days, while this group presented aspects of a mature connective tissue. FT-IR analysis showed a clear distinction among the three groups at all experimental times and groups T and I presented more similarities to each other than to group C. Conclusion: Acute injury of tendon treated with human amniotic membrane fragment showed a faster healing process, reduction in inflammatory response, intense proliferation of fibroblasts and organization of collagen fibers.

Bibliographic Details

Main Authors:	Nicodemo,Mariana de Castro, Neves,Lia Renó das, Aguiar,Josafá Carvalho, Brito,Flaviane de Souza, Ferreira,Isabelle, Sant'Anna,Luciana Barros, Raniero,Leandro José, Martins,Rodrigo Álvaro Lopes, Barja,Paulo Roxo, Arisawa,Emilia Angela Lo Schiavo
Format:	Digital revista
Language:	English
Published:	Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia 2017
Online Access:	http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502017000200125
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