Sulfated fucan from marine alga inhibits HeLa cells infection by HTLV-1 free particles: semi-quantitative analysis

A sulfated fucan from Laminaria abyssalis marine alga prevented the interaction of HTLV-1 particles, purified from the MT-2 cell line, with HeLa cells. The infection obtained using a concentrated virus suspension was detected only by amplification of the newly synthesized HTLV-1 proviral cDNA by the nested-polymerase chain reaction (PCR). The sulfated polysaccharide was not toxic to the cells at a concentration of 100 µg/mL and prevented infection by the viral particles when added to the cell monolayers. The proviral cDNA was only detected when the sulfated polysaccharide was added to the cells three hours post-infection, indicating that the inhibitory activity occurred in the initial stages of virus-cell interaction. Our results demonstrate, for the first time, the ability of a sulfated fucan from marine algae to inhibit virus transmission through free virus particles.

Saved in:
Bibliographic Details
Main Authors: Romanos,Maria T. V., Andrada-Serpa,Maria J., Mourão,Paulo A. S., Yoneshigue-Valentin,Yocie, Pereira,Mariana S., Santos,Norma, Wigg,Marcia D.
Format: Digital revista
Language:English
Published: Sociedade Brasileira de Farmacognosia 2011
Online Access:http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-695X2011000200005
Tags: Add Tag
No Tags, Be the first to tag this record!