In vitro Dendrobium nobile plant growth and rooting in different sucrose concentrations
Sucrose is a very important component in in vitro culture media, serving as a source of carbon and energy. In this paper, the rooting and in vitro growth of Dendrobium nobile Lindl (Orchidaceae) were studied using different sucrose concentrations (0 g L-1; 5 g L-1; 10 g L-1; 20 g L-1; 30 g L-1 and 60 g L-1), in a modified MS medium containing half the regular concentration of macronutrients at pH 5.8. Greater increases in plant height (4.21±0.6 cm) and high seedling multiplication (1:4) were observed in the 60 g L-1 sucrose treatment, even without the addition of plant hormones. Sucrose concentration in the culture medium did not influence in vitro plant rooting.
Main Authors: | , , , |
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Format: | Digital revista |
Language: | English |
Published: |
Associação Brasileira de Horticultura
2004
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Online Access: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362004000400023 |
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