Use of RAPD-PCR to identify true hybrid plants from crosses between closely related progenitors

Use of RAPD-PCR to identify true hybrid plants from crosses between closely related progenitors

RAPD-PCR molecular markers were used to identify common bean and soybean hybrid plants derived from crosses between closely related progenitors, with no apparent phenotypic differences. Primers OP-F12 and OP-0O3 were used to identify true hybrids derived from crosses between common bean cultivars Rudá (A 285) and AN 910408, and soybean cultivars Cristalina and Bossier, respectively. Each primer generated one polymorphic DNA band which was present in the male progenitor and absent in the female progenitor. As RAPD bands are normally inherited as dominant characters, the presence of these bands in the F1 plants confirmed their status.

Saved in:
Bibliographic Details
Main Authors: Alzate-Marin,Ana Lilia, Baía,Gilson Soares, Martins Filho,Sebastião, Paula Júnior,Trazilbo José de, Sediyama,Carlos Sigueyuki, Barros,Everaldo Gonçalves de, Moreira,Maurilio Alves
Format: Digital revista
Language:English
Published: Sociedade Brasileira de Genética 1996
Online Access:http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-84551996000400016
Tags: Add Tag
No Tags, Be the first to tag this record!

Internet

http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-84551996000400016

Similar Items