Evaluation of the indirect fluorescent antibody test and modified agglutination test for detection of antibodies against Toxoplasma gondii in experimentally infected pigs
The study determined the sensitivity and specificity of the indirect fluorescent antibody test (IFAT) and modified agglutination test (MAT) for anti-Toxoplasma gondii antibody detection by analyzing sera from 46 experimentally infected pigs. Values for sensitivity were 95.7% (confidence interval 95%: 84.0-99.2%) and for specificity 97.8% (confidence interval 95%: 87.0-99.9%) in both tests. There was an optimum agreement of results between IFAT and MAT evidenced by a Kappa test of 0.86. These results validate these tests for the detection of T. gondii infection in pigs. IFAT and MAT despite methodologies with different characteristics and readings have similar accuracy in pig serum samples.
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Colégio Brasileiro de Patologia Animal - CBPA
2004
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oai:scielo:S0100-736X20040004000052005-03-21Evaluation of the indirect fluorescent antibody test and modified agglutination test for detection of antibodies against Toxoplasma gondii in experimentally infected pigsMinho,Alessandro PelegrineFreire,Roberta LemosVidotto,OdilonGennari,Solange MariaMarana,Elizabete MarangoniGarcia,João LuisNavarro,Italmar Teodorico Toxoplasma gondii swine antibodies serology indirect fluorescent antibody test (IFAT) modified agglutination test (MAT) The study determined the sensitivity and specificity of the indirect fluorescent antibody test (IFAT) and modified agglutination test (MAT) for anti-Toxoplasma gondii antibody detection by analyzing sera from 46 experimentally infected pigs. Values for sensitivity were 95.7% (confidence interval 95%: 84.0-99.2%) and for specificity 97.8% (confidence interval 95%: 87.0-99.9%) in both tests. There was an optimum agreement of results between IFAT and MAT evidenced by a Kappa test of 0.86. These results validate these tests for the detection of T. gondii infection in pigs. IFAT and MAT despite methodologies with different characteristics and readings have similar accuracy in pig serum samples.info:eu-repo/semantics/openAccessColégio Brasileiro de Patologia Animal - CBPAPesquisa Veterinária Brasileira v.24 n.4 20042004-12-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2004000400005en10.1590/S0100-736X2004000400005 |
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Minho,Alessandro Pelegrine Freire,Roberta Lemos Vidotto,Odilon Gennari,Solange Maria Marana,Elizabete Marangoni Garcia,João Luis Navarro,Italmar Teodorico |
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Minho,Alessandro Pelegrine Freire,Roberta Lemos Vidotto,Odilon Gennari,Solange Maria Marana,Elizabete Marangoni Garcia,João Luis Navarro,Italmar Teodorico Evaluation of the indirect fluorescent antibody test and modified agglutination test for detection of antibodies against Toxoplasma gondii in experimentally infected pigs |
| author_facet |
Minho,Alessandro Pelegrine Freire,Roberta Lemos Vidotto,Odilon Gennari,Solange Maria Marana,Elizabete Marangoni Garcia,João Luis Navarro,Italmar Teodorico |
| author_sort |
Minho,Alessandro Pelegrine |
| title |
Evaluation of the indirect fluorescent antibody test and modified agglutination test for detection of antibodies against Toxoplasma gondii in experimentally infected pigs |
| title_short |
Evaluation of the indirect fluorescent antibody test and modified agglutination test for detection of antibodies against Toxoplasma gondii in experimentally infected pigs |
| title_full |
Evaluation of the indirect fluorescent antibody test and modified agglutination test for detection of antibodies against Toxoplasma gondii in experimentally infected pigs |
| title_fullStr |
Evaluation of the indirect fluorescent antibody test and modified agglutination test for detection of antibodies against Toxoplasma gondii in experimentally infected pigs |
| title_full_unstemmed |
Evaluation of the indirect fluorescent antibody test and modified agglutination test for detection of antibodies against Toxoplasma gondii in experimentally infected pigs |
| title_sort |
evaluation of the indirect fluorescent antibody test and modified agglutination test for detection of antibodies against toxoplasma gondii in experimentally infected pigs |
| description |
The study determined the sensitivity and specificity of the indirect fluorescent antibody test (IFAT) and modified agglutination test (MAT) for anti-Toxoplasma gondii antibody detection by analyzing sera from 46 experimentally infected pigs. Values for sensitivity were 95.7% (confidence interval 95%: 84.0-99.2%) and for specificity 97.8% (confidence interval 95%: 87.0-99.9%) in both tests. There was an optimum agreement of results between IFAT and MAT evidenced by a Kappa test of 0.86. These results validate these tests for the detection of T. gondii infection in pigs. IFAT and MAT despite methodologies with different characteristics and readings have similar accuracy in pig serum samples. |
| publisher |
Colégio Brasileiro de Patologia Animal - CBPA |
| publishDate |
2004 |
| url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2004000400005 |
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