Evaluation of the indirect fluorescent antibody test and modified agglutination test for detection of antibodies against Toxoplasma gondii in experimentally infected pigs

The study determined the sensitivity and specificity of the indirect fluorescent antibody test (IFAT) and modified agglutination test (MAT) for anti-Toxoplasma gondii antibody detection by analyzing sera from 46 experimentally infected pigs. Values for sensitivity were 95.7% (confidence interval 95%: 84.0-99.2%) and for specificity 97.8% (confidence interval 95%: 87.0-99.9%) in both tests. There was an optimum agreement of results between IFAT and MAT evidenced by a Kappa test of 0.86. These results validate these tests for the detection of T. gondii infection in pigs. IFAT and MAT despite methodologies with different characteristics and readings have similar accuracy in pig serum samples.

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Bibliographic Details
Main Authors: Minho,Alessandro Pelegrine, Freire,Roberta Lemos, Vidotto,Odilon, Gennari,Solange Maria, Marana,Elizabete Marangoni, Garcia,João Luis, Navarro,Italmar Teodorico
Format: Digital revista
Language:English
Published: Colégio Brasileiro de Patologia Animal - CBPA 2004
Online Access:http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2004000400005
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