Spectrophotometric determination of nevirapine using tetrathiocyanatocobalt(II) ion as a reagent
A simple and rapid spectrophotometric method for the determination of nevirapine is described. The method is based on the reaction of nevirapine with tetrathiocyanatocobalt(II) ion in buffer of pH 4 to form the corresponding complex. Beer's law is obeyed in the range of 0.2 - 2.0 µg mL-1 for nevirapine. The optical parameters such as molar absorptivity, Sandell's sensitivity, detection limit and quantitation limit were found to be 1.16× 10(4) Lmol-1cm-1, 2.09 X 10-3 µg cm-2, 0.073 µg mL-1 and 0.222 µg mL-1 respectively. The optimum reaction conditions and other analytical parameters were evaluated. The statistical evaluation of the method was examined by determining intra-day and inter-day precision. The proposed method has been successfully applied for the determination of nevirapine in pharmaceutical formulations.
| Main Authors: | , |
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| Format: | Digital revista |
| Language: | English |
| Published: |
Fundação Editora da Universidade Estadual Paulista Júlio de Mesquita Filho - UNESP
2010
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| Online Access: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-46702010000300008 |
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