Quantificação sérica de vitamina C por CLAE-UV e estudo de estabilidade
Vitamin C, an exogenous antioxidant, is essential to human health. In this study, a method was validated to serum vitamin C quantification by HPLC-UV. Its stability with and without the use of tris [2-carboxy-ethyl] phosphine hydrochloride (TCEP), at -20 and -80 °C, in serum and supernatant were also evaluated. Analysis showed r² > 0.99, precision CV% < 15% and % bias < 15%, being linear, precise and accurate. The stability test revealed that using TCEP in serum storage at -20 and -80 °C or in supernatant at -80 °C the vitamin C levels remain stable for 30 and 12 days, respectively.
Saved in:
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Digital revista |
| Language: | Portuguese |
| Published: |
Sociedade Brasileira de Química
2012
|
| Online Access: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422012000200030 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|