Otimização e validação de método multirresíduo para determinação de sulfonamidas em camarão cultivado por cromatografia líquida de alta eficiência com detecção por UV

This work reports the optimization and method validation for sulfonamides (sulfamethazine, sulfaquinoxaline, sulfadimethoxine and sulfathiazole) in shrimp muscle using HPLC-UV. The sulfonamides were extracted with acetonitrile and acetic acid, and the extract cleaned up with a Strata SCX SPE cartridge prior to analysis. The method presented linearity in the range of 20-120 µg kg-1, good linear correlation (r > 0.99), and limits of quantification in the range of 4.7-20.2 µg kg-1. The recovery for shrimp muscles spiked with 50-150 µg kg-1 ranged from 63.2-108.0%. Precision and accuracy analysis showed acceptable relative standard deviation. Commercial shrimps were analyzed and sulfonamides don't were found above of the method limit of quantification.

Bibliographic Details

Main Authors:	Santos,Paloma Nascimento dos, Maciel,Maria Inês Sucupira, Lavorante,Beatriz Regina Brito de Oliveira, Medeiros,Marcelo de Mélo, Albuquerque Júnior,Eden Cavalcanti de
Format:	Digital revista
Language:	Portuguese
Published:	Sociedade Brasileira de Química 2011
Online Access:	http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422011000700027
Tags:	Add Tag No Tags, Be the first to tag this record!