In vitro organogenesis as an efficient method for the propagation of Dalbergia nigra
Abstract The objective of this work was to establish an efficient protocol for the in vitro organogenesis of Dalbergia nigra. For this purpose, 30-day-old seedlings were sectioned at their cotyledonary nodes and nodal segments. These materials were cultivated in a medium with different combinations of the 6-benzylaminopurine and thidiazuron cytokinins. After 60 days in a growth chamber set at 27°C and a 16-hour photoperiod, growth characteristics were analyzed. Explants from the cotyledonary nodes show a greater morphogenetic potential, regardless of the addition of cytokinins. Cotyledonary nodes in the medium supplemented with 2.22 µmol L-1 BAP show the best combination for the in vitro propagation of D. nigra.
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Embrapa Secretaria de Pesquisa e Desenvolvimento
2022
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oai:scielo:S0100-204X20220001017042022-12-01In vitro organogenesis as an efficient method for the propagation of Dalbergia nigraSimões,Ingridh MedeirosAraujo,Caroline Palacio deMello,Tamyris deRosa,Thuanny Lins MonteiroOliveira,Natasha Vieira deCaldeira,Marcos Vinícius WincklerSchmildt,Edilson RomaisLopes,José CarlosOtoni,Wagner CamposAlexandre,Rodrigo Sobreira Dalbergia nigra Brazilian rosewood explants in vitro culture 6-benzylaminopurine thidiazuron Abstract The objective of this work was to establish an efficient protocol for the in vitro organogenesis of Dalbergia nigra. For this purpose, 30-day-old seedlings were sectioned at their cotyledonary nodes and nodal segments. These materials were cultivated in a medium with different combinations of the 6-benzylaminopurine and thidiazuron cytokinins. After 60 days in a growth chamber set at 27°C and a 16-hour photoperiod, growth characteristics were analyzed. Explants from the cotyledonary nodes show a greater morphogenetic potential, regardless of the addition of cytokinins. Cotyledonary nodes in the medium supplemented with 2.22 µmol L-1 BAP show the best combination for the in vitro propagation of D. nigra.info:eu-repo/semantics/openAccessEmbrapa Secretaria de Pesquisa e DesenvolvimentoPesquisa Agropecuária BrasileiraPesquisa Agropecuária Brasileira v.57 20222022-01-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2022000101704en10.1590/s1678-3921.pab2022.v57.02766 |
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Simões,Ingridh Medeiros Araujo,Caroline Palacio de Mello,Tamyris de Rosa,Thuanny Lins Monteiro Oliveira,Natasha Vieira de Caldeira,Marcos Vinícius Winckler Schmildt,Edilson Romais Lopes,José Carlos Otoni,Wagner Campos Alexandre,Rodrigo Sobreira |
| spellingShingle |
Simões,Ingridh Medeiros Araujo,Caroline Palacio de Mello,Tamyris de Rosa,Thuanny Lins Monteiro Oliveira,Natasha Vieira de Caldeira,Marcos Vinícius Winckler Schmildt,Edilson Romais Lopes,José Carlos Otoni,Wagner Campos Alexandre,Rodrigo Sobreira In vitro organogenesis as an efficient method for the propagation of Dalbergia nigra |
| author_facet |
Simões,Ingridh Medeiros Araujo,Caroline Palacio de Mello,Tamyris de Rosa,Thuanny Lins Monteiro Oliveira,Natasha Vieira de Caldeira,Marcos Vinícius Winckler Schmildt,Edilson Romais Lopes,José Carlos Otoni,Wagner Campos Alexandre,Rodrigo Sobreira |
| author_sort |
Simões,Ingridh Medeiros |
| title |
In vitro organogenesis as an efficient method for the propagation of Dalbergia nigra |
| title_short |
In vitro organogenesis as an efficient method for the propagation of Dalbergia nigra |
| title_full |
In vitro organogenesis as an efficient method for the propagation of Dalbergia nigra |
| title_fullStr |
In vitro organogenesis as an efficient method for the propagation of Dalbergia nigra |
| title_full_unstemmed |
In vitro organogenesis as an efficient method for the propagation of Dalbergia nigra |
| title_sort |
in vitro organogenesis as an efficient method for the propagation of dalbergia nigra |
| description |
Abstract The objective of this work was to establish an efficient protocol for the in vitro organogenesis of Dalbergia nigra. For this purpose, 30-day-old seedlings were sectioned at their cotyledonary nodes and nodal segments. These materials were cultivated in a medium with different combinations of the 6-benzylaminopurine and thidiazuron cytokinins. After 60 days in a growth chamber set at 27°C and a 16-hour photoperiod, growth characteristics were analyzed. Explants from the cotyledonary nodes show a greater morphogenetic potential, regardless of the addition of cytokinins. Cotyledonary nodes in the medium supplemented with 2.22 µmol L-1 BAP show the best combination for the in vitro propagation of D. nigra. |
| publisher |
Embrapa Secretaria de Pesquisa e Desenvolvimento |
| publishDate |
2022 |
| url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2022000101704 |
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