In vitro organogenesis as an efficient method for the propagation of Dalbergia nigra

Abstract The objective of this work was to establish an efficient protocol for the in vitro organogenesis of Dalbergia nigra. For this purpose, 30-day-old seedlings were sectioned at their cotyledonary nodes and nodal segments. These materials were cultivated in a medium with different combinations of the 6-benzylaminopurine and thidiazuron cytokinins. After 60 days in a growth chamber set at 27°C and a 16-hour photoperiod, growth characteristics were analyzed. Explants from the cotyledonary nodes show a greater morphogenetic potential, regardless of the addition of cytokinins. Cotyledonary nodes in the medium supplemented with 2.22 µmol L-1 BAP show the best combination for the in vitro propagation of D. nigra.

Bibliographic Details

Main Authors:	Simões,Ingridh Medeiros, Araujo,Caroline Palacio de, Mello,Tamyris de, Rosa,Thuanny Lins Monteiro, Oliveira,Natasha Vieira de, Caldeira,Marcos Vinícius Winckler, Schmildt,Edilson Romais, Lopes,José Carlos, Otoni,Wagner Campos, Alexandre,Rodrigo Sobreira
Format:	Digital revista
Language:	English
Published:	Embrapa Secretaria de Pesquisa e Desenvolvimento 2022
Online Access:	http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2022000101704
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