Miltefosine induces programmed cell death in Leishmania amazonensis promastigotes

In the current study, we evaluated the mechanism of action of miltefosine, which is the first effective and safe oral treatment for visceral leishmaniasis, in Leishmania amazonensis promastigotes. Miltefosine induced a process of programmed cell death, which was determined by the externalization of phosphatidylserine, the incorporation of propidium iodide, cell-cycle arrest at the sub-G0/G1 phase and DNA fragmentation into oligonucleosome-sized fragments. Despite the intrinsic variation that is detected in Leishmania spp, our results indicate that miltefosine causes apoptosis-like death in L. amazonensis promastigote cells using a similar process that is observed in Leishmania donovani.

Bibliographic Details

Main Authors:	Marinho,Fernanda de Aquino, Gonçalves,Keyla Cristiny da Silva, Oliveira,Selma Soares de, Oliveira,Ana-Carolina de Siqueira Couto de, Bellio,Maria, d'Avila-Levy,Claudia Masini, Santos,André Luis Souza dos, Branquinha,Marta Helena
Format:	Digital revista
Language:	English
Published:	Instituto Oswaldo Cruz, Ministério da Saúde 2011
Online Access:	http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762011000400021
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