Detection of Lymnaea columella infection by Fasciola hepatica through Multiplex-PCR
From complete mitochondrial DNA sequence of Fasciola hepatica available in Genbank, specific primers were designed for a conserved and repetitive region of this trematode. A pair of primers was used for diagnosis of infected Lymnaea columella by F. hepatica during the pre-patent period simultaneously with another pair of primers which amplified the internal transcribed spacer (ITS) region of rDNA from L. columella in a single Multiplex-PCR. The amplification generated a ladder band profile specific for F. hepatica. This profile was observed in positive molluscs at different times of infection, including adult worms from the trematode. The Multiplex-PCR technique showed to be a fast and safe tool for fascioliasis diagnosis, enabling the detection of F. hepatica miracidia in L. columella during the pre-patent period and identification of transmission areas.
| Main Authors: | , , |
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| Format: | Digital revista |
| Language: | English |
| Published: |
Instituto Oswaldo Cruz, Ministério da Saúde
2004
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| Online Access: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762004000400013 |
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