Standardization of bovine macrophage monolayers and isolation and culture of trypanosomes

Standardization of bovine macrophage monolayers and isolation and culture of trypanosomes

We describe a method for culturing over 90% pure bovine macrophages from peripheral blood mononuclear cells separated with Nycoprep. The cells were cultured for 12 days and then stained with esterase and with anti CD14 to test for purity. The method is reproducible and ensures an adequate number of cells for immunological research. Additionally, we report the unexpected finding of Trypanosoma trypomastigotes in our macrophage cultures from bovines belonging to a geographic area from which no bovine trypanosomes had been reported before.

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Main Authors: Saldarriaga,Omar A, Velásquez,Jaime I, Ossa,Jorge E, Rugeles,Maria T
Format: Digital revista
Language:English
Published: Instituto Oswaldo Cruz, Ministério da Saúde 2003
Online Access:http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762003000200017
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spelling oai:scielo:S0074-027620030002000172003-05-20Standardization of bovine macrophage monolayers and isolation and culture of trypanosomesSaldarriaga,Omar AVelásquez,Jaime IOssa,Jorge ERugeles,Maria T macrophages bovine trypanosomes We describe a method for culturing over 90% pure bovine macrophages from peripheral blood mononuclear cells separated with Nycoprep. The cells were cultured for 12 days and then stained with esterase and with anti CD14 to test for purity. The method is reproducible and ensures an adequate number of cells for immunological research. Additionally, we report the unexpected finding of Trypanosoma trypomastigotes in our macrophage cultures from bovines belonging to a geographic area from which no bovine trypanosomes had been reported before.info:eu-repo/semantics/openAccessInstituto Oswaldo Cruz, Ministério da SaúdeMemórias do Instituto Oswaldo Cruz v.98 n.2 20032003-03-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762003000200017en10.1590/S0074-02762003000200017
institution SCIELO
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country Brasil
countrycode BR
component Revista
access En linea
databasecode rev-scielo-br
tag revista
region America del Sur
libraryname SciELO
language English
format Digital
author Saldarriaga,Omar A
Velásquez,Jaime I
Ossa,Jorge E
Rugeles,Maria T
spellingShingle Saldarriaga,Omar A
Velásquez,Jaime I
Ossa,Jorge E
Rugeles,Maria T
Standardization of bovine macrophage monolayers and isolation and culture of trypanosomes
author_facet Saldarriaga,Omar A
Velásquez,Jaime I
Ossa,Jorge E
Rugeles,Maria T
author_sort Saldarriaga,Omar A
title Standardization of bovine macrophage monolayers and isolation and culture of trypanosomes
title_short Standardization of bovine macrophage monolayers and isolation and culture of trypanosomes
title_full Standardization of bovine macrophage monolayers and isolation and culture of trypanosomes
title_fullStr Standardization of bovine macrophage monolayers and isolation and culture of trypanosomes
title_full_unstemmed Standardization of bovine macrophage monolayers and isolation and culture of trypanosomes
title_sort standardization of bovine macrophage monolayers and isolation and culture of trypanosomes
description We describe a method for culturing over 90% pure bovine macrophages from peripheral blood mononuclear cells separated with Nycoprep. The cells were cultured for 12 days and then stained with esterase and with anti CD14 to test for purity. The method is reproducible and ensures an adequate number of cells for immunological research. Additionally, we report the unexpected finding of Trypanosoma trypomastigotes in our macrophage cultures from bovines belonging to a geographic area from which no bovine trypanosomes had been reported before.
publisher Instituto Oswaldo Cruz, Ministério da Saúde
publishDate 2003
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762003000200017
work_keys_str_mv AT saldarriagaomara standardizationofbovinemacrophagemonolayersandisolationandcultureoftrypanosomes
AT velasquezjaimei standardizationofbovinemacrophagemonolayersandisolationandcultureoftrypanosomes
AT ossajorgee standardizationofbovinemacrophagemonolayersandisolationandcultureoftrypanosomes
AT rugelesmariat standardizationofbovinemacrophagemonolayersandisolationandcultureoftrypanosomes
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