Standardization of bovine macrophage monolayers and isolation and culture of trypanosomes
We describe a method for culturing over 90% pure bovine macrophages from peripheral blood mononuclear cells separated with Nycoprep. The cells were cultured for 12 days and then stained with esterase and with anti CD14 to test for purity. The method is reproducible and ensures an adequate number of cells for immunological research. Additionally, we report the unexpected finding of Trypanosoma trypomastigotes in our macrophage cultures from bovines belonging to a geographic area from which no bovine trypanosomes had been reported before.
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Digital revista |
| Language: | English |
| Published: |
Instituto Oswaldo Cruz, Ministério da Saúde
2003
|
| Online Access: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762003000200017 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|