A simple method for assessing the binding of concanavalin A to mononuclear cell surfaces: no interference of visceral leishmaniasis serum on this binding

We report a simple method for evaluating the binding of concanavalin A (ConA) to human peripheral blood mononuclear cells (PBMC). The binding is evidenced by an immunoenzymic assay using peroxidase-conjugated immunoglobulins of a rabbit anti-ConA serum. Using the method we show that sera from patients with American leishmaniasis do not interfere with binding of ConA to PBMC.

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Bibliographic Details
Main Authors: Barral-Netto,Manoel, Barral,A.
Format: Digital revista
Language:English
Published: Instituto Oswaldo Cruz, Ministério da Saúde 1986
Online Access:http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761986000300012
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