Cryptosporidium diagnosis in different groups of children and characterization of parasite species

ABSTRACT Background: Microscopy and enzyme-linked immunosorbent assay (ELISA) are routinely used for Cryptosporidium diagnosis, without differentiating the parasite species. Methods: Children’s feces were analyzed by modified Ziehl-Neelsen (mZN) and ELISA for Cryptosporidium diagnosis and by polymerase chain reaction-restriction fragment length polymorphism for species identification. Results: Cryptosporidium frequency was 2.6%. The sensitivity and specificity of ELISA were 85.7% and 99.7%, respectively, with excellent concordance with mZN (kappa=0.854). Parasite species were characterized as Cryptosporidium hominis (78.3%), Cryptosporidium felis (17.4%), and Cryptosporidium parvum (4.3%). Conclusions: Coproantigen ELISA is as efficient as mZN for Cryptosporidium diagnosis. Cryptosporidium genotyping suggests anthroponotic and zoonotic transmission to children.

Bibliographic Details

Main Authors:	Pacheco,Flávia Thamiris Figueiredo, Freitas,Humberto Fonseca de, Silva,Renata Kelly Novais Rodrigues, Carvalho,Silvia Souza de, Martins,Adson Santos, Menezes,Joelma Figueiredo, Ribeiro,Tereza Cristina Medrado, Mattos,Ângela Peixoto de, Costa-Ribeiro Júnior,Hugo da, Pedreira,Joice Neves Reis, Soares,Neci Matos, Teixeira,Márcia Cristina Aquino
Format:	Digital revista
Language:	English
Published:	Sociedade Brasileira de Medicina Tropical - SBMT 2022
Online Access:	http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822022000100616
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