Filter paper performance in PCR for cutaneous leishmaniasis diagnosis

Filter paper performance in PCR for cutaneous leishmaniasis diagnosis

Abstract INTRODUCTION: The objective of this study was to evaluate the performance of filter paper (FP) for lesion scraping collection in a polymerase chain reaction (PCR) for cutaneous leishmaniasis (CL) diagnosis. METHODS: Lesion scrapings from 48 patients were collected and analyzed for PCR. RESULTS: PCR with FP detected up to three Leishmania braziliensis promastigotes. Considering the direct search by microscopy or PCR of samples collected in STE buffer as standards, the sensitivity of PCR with FP was 100%. CONCLUSIONS: FP can be useful for CL diagnosis in remote regions, allowing high sensitivity in the detection of the parasite by PCR.

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Bibliographic Details
Main Authors: Mota,Camila Alves, Venazzi,Eneide Aparecida Sabaini, Zanzarini,Paulo Donizeti, Aristides,Sandra Mara Alessi, Lonardoni,Maria Valdrinez Campana, Silveira,Thaís Gomes Verzignassi
Format: Digital revista
Language:English
Published: Sociedade Brasileira de Medicina Tropical - SBMT 2021
Online Access:http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822021000100605
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