Can Aspergillus fumigatus conidia cause false-positive results in the galactomannan enzyme immunoassay test?

Can Aspergillus fumigatus conidia cause false-positive results in the galactomannan enzyme immunoassay test?

Abstract INTRODUCTION: Several factors can cause false-positive results in the galactomannan (GM) test; however, others remain unknown. Presently, the impact of airborne contamination by Aspergillus conidia during enzyme-linked immunosorbent assay (ELISA) remains uninvestigated. METHODS: We studied 12 A. fumigatus isolates. Fungal conidia were serially diluted and tested for GM detection using the Platelia® Aspergillus enzyme immunoassay (EIA). RESULTS: The conidia concentration required for an EIA-positive result was 4.8 × 103 (median). CONCLUSIONS: This is the first study to evaluate the impact of environmental contamination on the Platelia® Aspergillus EIA assay. Only massive contamination can interfere with GM optical readings, suggesting that environmental contamination does not cause false-positive test results.

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Main Authors: Cabana,Ângela Leitzke, Mendes,Josiara Furtado, Klafke,Gabriel Baracy, Brandolt,Tchana Martinez, Melo,Aryse Martins, Meireles,Mário Carlos Araújo, Xavier,Melissa Orzechowski
Format: Digital revista
Language:English
Published: Sociedade Brasileira de Medicina Tropical - SBMT 2018
Online Access:http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822018000300387
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spelling oai:scielo:S0037-868220180003003872018-12-04Can Aspergillus fumigatus conidia cause false-positive results in the galactomannan enzyme immunoassay test?Cabana,Ângela LeitzkeMendes,Josiara FurtadoKlafke,Gabriel BaracyBrandolt,Tchana MartinezMelo,Aryse MartinsMeireles,Mário Carlos AraújoXavier,Melissa Orzechowski Invasive aspergillosis Diagnosis Platelia® Aspergillus EIA Galactomannan Air quality Abstract INTRODUCTION: Several factors can cause false-positive results in the galactomannan (GM) test; however, others remain unknown. Presently, the impact of airborne contamination by Aspergillus conidia during enzyme-linked immunosorbent assay (ELISA) remains uninvestigated. METHODS: We studied 12 A. fumigatus isolates. Fungal conidia were serially diluted and tested for GM detection using the Platelia® Aspergillus enzyme immunoassay (EIA). RESULTS: The conidia concentration required for an EIA-positive result was 4.8 × 103 (median). CONCLUSIONS: This is the first study to evaluate the impact of environmental contamination on the Platelia® Aspergillus EIA assay. Only massive contamination can interfere with GM optical readings, suggesting that environmental contamination does not cause false-positive test results.info:eu-repo/semantics/openAccessSociedade Brasileira de Medicina Tropical - SBMTRevista da Sociedade Brasileira de Medicina Tropical v.51 n.3 20182018-06-01info:eu-repo/semantics/othertext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822018000300387en10.1590/0037-8682-0317-2017
institution SCIELO
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country Brasil
countrycode BR
component Revista
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databasecode rev-scielo-br
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region America del Sur
libraryname SciELO
language English
format Digital
author Cabana,Ângela Leitzke
Mendes,Josiara Furtado
Klafke,Gabriel Baracy
Brandolt,Tchana Martinez
Melo,Aryse Martins
Meireles,Mário Carlos Araújo
Xavier,Melissa Orzechowski
spellingShingle Cabana,Ângela Leitzke
Mendes,Josiara Furtado
Klafke,Gabriel Baracy
Brandolt,Tchana Martinez
Melo,Aryse Martins
Meireles,Mário Carlos Araújo
Xavier,Melissa Orzechowski
Can Aspergillus fumigatus conidia cause false-positive results in the galactomannan enzyme immunoassay test?
author_facet Cabana,Ângela Leitzke
Mendes,Josiara Furtado
Klafke,Gabriel Baracy
Brandolt,Tchana Martinez
Melo,Aryse Martins
Meireles,Mário Carlos Araújo
Xavier,Melissa Orzechowski
author_sort Cabana,Ângela Leitzke
title Can Aspergillus fumigatus conidia cause false-positive results in the galactomannan enzyme immunoassay test?
title_short Can Aspergillus fumigatus conidia cause false-positive results in the galactomannan enzyme immunoassay test?
title_full Can Aspergillus fumigatus conidia cause false-positive results in the galactomannan enzyme immunoassay test?
title_fullStr Can Aspergillus fumigatus conidia cause false-positive results in the galactomannan enzyme immunoassay test?
title_full_unstemmed Can Aspergillus fumigatus conidia cause false-positive results in the galactomannan enzyme immunoassay test?
title_sort can aspergillus fumigatus conidia cause false-positive results in the galactomannan enzyme immunoassay test?
description Abstract INTRODUCTION: Several factors can cause false-positive results in the galactomannan (GM) test; however, others remain unknown. Presently, the impact of airborne contamination by Aspergillus conidia during enzyme-linked immunosorbent assay (ELISA) remains uninvestigated. METHODS: We studied 12 A. fumigatus isolates. Fungal conidia were serially diluted and tested for GM detection using the Platelia® Aspergillus enzyme immunoassay (EIA). RESULTS: The conidia concentration required for an EIA-positive result was 4.8 × 103 (median). CONCLUSIONS: This is the first study to evaluate the impact of environmental contamination on the Platelia® Aspergillus EIA assay. Only massive contamination can interfere with GM optical readings, suggesting that environmental contamination does not cause false-positive test results.
publisher Sociedade Brasileira de Medicina Tropical - SBMT
publishDate 2018
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822018000300387
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