Can Aspergillus fumigatus conidia cause false-positive results in the galactomannan enzyme immunoassay test?

Abstract INTRODUCTION: Several factors can cause false-positive results in the galactomannan (GM) test; however, others remain unknown. Presently, the impact of airborne contamination by Aspergillus conidia during enzyme-linked immunosorbent assay (ELISA) remains uninvestigated. METHODS: We studied 12 A. fumigatus isolates. Fungal conidia were serially diluted and tested for GM detection using the Platelia® Aspergillus enzyme immunoassay (EIA). RESULTS: The conidia concentration required for an EIA-positive result was 4.8 × 103 (median). CONCLUSIONS: This is the first study to evaluate the impact of environmental contamination on the Platelia® Aspergillus EIA assay. Only massive contamination can interfere with GM optical readings, suggesting that environmental contamination does not cause false-positive test results.

Bibliographic Details

Main Authors:	Cabana,Ângela Leitzke, Mendes,Josiara Furtado, Klafke,Gabriel Baracy, Brandolt,Tchana Martinez, Melo,Aryse Martins, Meireles,Mário Carlos Araújo, Xavier,Melissa Orzechowski
Format:	Digital revista
Language:	English
Published:	Sociedade Brasileira de Medicina Tropical - SBMT 2018
Online Access:	http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822018000300387
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