Detection of Leishmania (Viannia) DNA in leucocytes from the blood of patients with cutaneous leishmaniasis
ABSTRACTINTRODUCTION:Cutaneous leishmaniasis (CL) is a serious and global public health issue, with the potential of developing a mucosal form, occurring as subclinical cases, and showing recurrence despite previous treatment.METHODS:Polymorphonuclear and mononuclear DNA obtained from 49 patients was subjected to polymerase chain reaction for detection of Leishmania (Viannia).RESULTS:DNA was detected in mononuclear cells from two patients with active primary lesions positive for CL, with infection periods of 3 and 6 months, respectively.CONCLUSIONS:The DNA of Leishmania (Viannia) indicates probable parasite dissemination possibly explaining subclinical case emergence, lesion recurrence, and mucosal lesion appearance.
Main Authors: | , , , , , |
---|---|
Format: | Digital revista |
Language: | English |
Published: |
Sociedade Brasileira de Medicina Tropical - SBMT
2015
|
Online Access: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822015000500626 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
id |
oai:scielo:S0037-86822015000500626 |
---|---|
record_format |
ojs |
spelling |
oai:scielo:S0037-868220150005006262015-10-20Detection of Leishmania (Viannia) DNA in leucocytes from the blood of patients with cutaneous leishmaniasisConter,Carolina CellaNeitzke-Abreu,Herintha CoetoPedroso,Raissa BocchiLonardoni,Maria Valdrinez CampanaSilveira,Thaís Gomes VerzignassiAristides,Sandra Mara Alessi Polymerase chain reaction Leishmania ( Viannia ) Leucocyte ABSTRACTINTRODUCTION:Cutaneous leishmaniasis (CL) is a serious and global public health issue, with the potential of developing a mucosal form, occurring as subclinical cases, and showing recurrence despite previous treatment.METHODS:Polymorphonuclear and mononuclear DNA obtained from 49 patients was subjected to polymerase chain reaction for detection of Leishmania (Viannia).RESULTS:DNA was detected in mononuclear cells from two patients with active primary lesions positive for CL, with infection periods of 3 and 6 months, respectively.CONCLUSIONS:The DNA of Leishmania (Viannia) indicates probable parasite dissemination possibly explaining subclinical case emergence, lesion recurrence, and mucosal lesion appearance.info:eu-repo/semantics/openAccessSociedade Brasileira de Medicina Tropical - SBMTRevista da Sociedade Brasileira de Medicina Tropical v.48 n.5 20152015-10-01info:eu-repo/semantics/othertext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822015000500626en10.1590/0037-8682-0052-2015 |
institution |
SCIELO |
collection |
OJS |
country |
Brasil |
countrycode |
BR |
component |
Revista |
access |
En linea |
databasecode |
rev-scielo-br |
tag |
revista |
region |
America del Sur |
libraryname |
SciELO |
language |
English |
format |
Digital |
author |
Conter,Carolina Cella Neitzke-Abreu,Herintha Coeto Pedroso,Raissa Bocchi Lonardoni,Maria Valdrinez Campana Silveira,Thaís Gomes Verzignassi Aristides,Sandra Mara Alessi |
spellingShingle |
Conter,Carolina Cella Neitzke-Abreu,Herintha Coeto Pedroso,Raissa Bocchi Lonardoni,Maria Valdrinez Campana Silveira,Thaís Gomes Verzignassi Aristides,Sandra Mara Alessi Detection of Leishmania (Viannia) DNA in leucocytes from the blood of patients with cutaneous leishmaniasis |
author_facet |
Conter,Carolina Cella Neitzke-Abreu,Herintha Coeto Pedroso,Raissa Bocchi Lonardoni,Maria Valdrinez Campana Silveira,Thaís Gomes Verzignassi Aristides,Sandra Mara Alessi |
author_sort |
Conter,Carolina Cella |
title |
Detection of Leishmania (Viannia) DNA in leucocytes from the blood of patients with cutaneous leishmaniasis |
title_short |
Detection of Leishmania (Viannia) DNA in leucocytes from the blood of patients with cutaneous leishmaniasis |
title_full |
Detection of Leishmania (Viannia) DNA in leucocytes from the blood of patients with cutaneous leishmaniasis |
title_fullStr |
Detection of Leishmania (Viannia) DNA in leucocytes from the blood of patients with cutaneous leishmaniasis |
title_full_unstemmed |
Detection of Leishmania (Viannia) DNA in leucocytes from the blood of patients with cutaneous leishmaniasis |
title_sort |
detection of leishmania (viannia) dna in leucocytes from the blood of patients with cutaneous leishmaniasis |
description |
ABSTRACTINTRODUCTION:Cutaneous leishmaniasis (CL) is a serious and global public health issue, with the potential of developing a mucosal form, occurring as subclinical cases, and showing recurrence despite previous treatment.METHODS:Polymorphonuclear and mononuclear DNA obtained from 49 patients was subjected to polymerase chain reaction for detection of Leishmania (Viannia).RESULTS:DNA was detected in mononuclear cells from two patients with active primary lesions positive for CL, with infection periods of 3 and 6 months, respectively.CONCLUSIONS:The DNA of Leishmania (Viannia) indicates probable parasite dissemination possibly explaining subclinical case emergence, lesion recurrence, and mucosal lesion appearance. |
publisher |
Sociedade Brasileira de Medicina Tropical - SBMT |
publishDate |
2015 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822015000500626 |
work_keys_str_mv |
AT contercarolinacella detectionofleishmaniavianniadnainleucocytesfromthebloodofpatientswithcutaneousleishmaniasis AT neitzkeabreuherinthacoeto detectionofleishmaniavianniadnainleucocytesfromthebloodofpatientswithcutaneousleishmaniasis AT pedrosoraissabocchi detectionofleishmaniavianniadnainleucocytesfromthebloodofpatientswithcutaneousleishmaniasis AT lonardonimariavaldrinezcampana detectionofleishmaniavianniadnainleucocytesfromthebloodofpatientswithcutaneousleishmaniasis AT silveirathaisgomesverzignassi detectionofleishmaniavianniadnainleucocytesfromthebloodofpatientswithcutaneousleishmaniasis AT aristidessandramaraalessi detectionofleishmaniavianniadnainleucocytesfromthebloodofpatientswithcutaneousleishmaniasis |
_version_ |
1756380563554435072 |