Phenotypic and genotypic profile of pyrethroid resistance in populations of the mosquito Aedes aegypti from Goiânia, Central West Brazil
ABSTRACTINTRODUCTION:The mosquito Aedes aegypti has evolved resistance to pyrethroid insecticides. The present study evaluated Ae. aegypti from Goiânia for the resistant phenotype and for mutations associated with resistance.METHODS:Insecticide dose-response bioassays were conducted on mosquitoes descended from field-collected eggs, and polymerase chain reaction (PCR) was used to genotype 90 individuals at sites implicated in pyrethroid resistance.RESULTS:All mosquito populations displayed high levels of resistance to deltamethrin, as well as high frequencies of the 1016Ile kdr and 1534Cys kdrmutations.CONCLUSIONS:Aedes aegypti populations in the Goiânia area are highly resistant to deltamethrin, presumably due to high frequencies of kdr(knockdown-resistance) mutations.
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Sociedade Brasileira de Medicina Tropical - SBMT
2015
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oai:scielo:S0037-868220150005006072015-10-20Phenotypic and genotypic profile of pyrethroid resistance in populations of the mosquito Aedes aegypti from Goiânia, Central West BrazilChapadense,Francesca Guaracyaba GarciaFernandes,Everton Kort KampLima,José Bento PereiraMartins,Ademir JesusSilva,Luana CarraraRocha,Welington Tristão daSantos,Adelair Helena dosCravo,Pedro Aedes aegypti Resistance Voltage-gated sodium channel ABSTRACTINTRODUCTION:The mosquito Aedes aegypti has evolved resistance to pyrethroid insecticides. The present study evaluated Ae. aegypti from Goiânia for the resistant phenotype and for mutations associated with resistance.METHODS:Insecticide dose-response bioassays were conducted on mosquitoes descended from field-collected eggs, and polymerase chain reaction (PCR) was used to genotype 90 individuals at sites implicated in pyrethroid resistance.RESULTS:All mosquito populations displayed high levels of resistance to deltamethrin, as well as high frequencies of the 1016Ile kdr and 1534Cys kdrmutations.CONCLUSIONS:Aedes aegypti populations in the Goiânia area are highly resistant to deltamethrin, presumably due to high frequencies of kdr(knockdown-resistance) mutations.info:eu-repo/semantics/openAccessSociedade Brasileira de Medicina Tropical - SBMTRevista da Sociedade Brasileira de Medicina Tropical v.48 n.5 20152015-10-01info:eu-repo/semantics/othertext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822015000500607en10.1590/0037-8682-0046-2015 |
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Chapadense,Francesca Guaracyaba Garcia Fernandes,Everton Kort Kamp Lima,José Bento Pereira Martins,Ademir Jesus Silva,Luana Carrara Rocha,Welington Tristão da Santos,Adelair Helena dos Cravo,Pedro |
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Chapadense,Francesca Guaracyaba Garcia Fernandes,Everton Kort Kamp Lima,José Bento Pereira Martins,Ademir Jesus Silva,Luana Carrara Rocha,Welington Tristão da Santos,Adelair Helena dos Cravo,Pedro Phenotypic and genotypic profile of pyrethroid resistance in populations of the mosquito Aedes aegypti from Goiânia, Central West Brazil |
author_facet |
Chapadense,Francesca Guaracyaba Garcia Fernandes,Everton Kort Kamp Lima,José Bento Pereira Martins,Ademir Jesus Silva,Luana Carrara Rocha,Welington Tristão da Santos,Adelair Helena dos Cravo,Pedro |
author_sort |
Chapadense,Francesca Guaracyaba Garcia |
title |
Phenotypic and genotypic profile of pyrethroid resistance in populations of the mosquito Aedes aegypti from Goiânia, Central West Brazil |
title_short |
Phenotypic and genotypic profile of pyrethroid resistance in populations of the mosquito Aedes aegypti from Goiânia, Central West Brazil |
title_full |
Phenotypic and genotypic profile of pyrethroid resistance in populations of the mosquito Aedes aegypti from Goiânia, Central West Brazil |
title_fullStr |
Phenotypic and genotypic profile of pyrethroid resistance in populations of the mosquito Aedes aegypti from Goiânia, Central West Brazil |
title_full_unstemmed |
Phenotypic and genotypic profile of pyrethroid resistance in populations of the mosquito Aedes aegypti from Goiânia, Central West Brazil |
title_sort |
phenotypic and genotypic profile of pyrethroid resistance in populations of the mosquito aedes aegypti from goiânia, central west brazil |
description |
ABSTRACTINTRODUCTION:The mosquito Aedes aegypti has evolved resistance to pyrethroid insecticides. The present study evaluated Ae. aegypti from Goiânia for the resistant phenotype and for mutations associated with resistance.METHODS:Insecticide dose-response bioassays were conducted on mosquitoes descended from field-collected eggs, and polymerase chain reaction (PCR) was used to genotype 90 individuals at sites implicated in pyrethroid resistance.RESULTS:All mosquito populations displayed high levels of resistance to deltamethrin, as well as high frequencies of the 1016Ile kdr and 1534Cys kdrmutations.CONCLUSIONS:Aedes aegypti populations in the Goiânia area are highly resistant to deltamethrin, presumably due to high frequencies of kdr(knockdown-resistance) mutations. |
publisher |
Sociedade Brasileira de Medicina Tropical - SBMT |
publishDate |
2015 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822015000500607 |
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