Rapid detection of Van genes in rectal swabs by real time PCR in Southern Brazil

Rapid detection of Van genes in rectal swabs by real time PCR in Southern Brazil

INTRODUCTION: Laboratory-based surveillance is an important component in the control of vancomycin resistant enterococci (VRE). METHODS: The study aimed to evaluate real-time polymerase chain reaction (RT-PCR) (genes vanA-vanB) for VRE detection on 115 swabs from patients included in a surveillance program. RESULTS: Sensitivity of RT-PCR was similar to primary culture (75% and 79.5%, respectively) when compared to broth enriched culture, whereas specificity was 83.1%. CONCLUSIONS: RT-PCR provides same day results, however it showed low sensitivity for VRE detection.

Saved in:
Bibliographic Details
Main Authors: Cantarelli,Vlademir, Cavalcante,Bianca, Pilger,Diogo André, Souza,Fabiana, Dias,Cícero Gomes, Brodt,Teresa, Cantarelli,Maritza, Secchi,Carina, d'Azevedo,Pedro Alves
Format: Digital revista
Language:English
Published: Sociedade Brasileira de Medicina Tropical - SBMT 2011
Online Access:http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822011000500021
Tags: Add Tag
No Tags, Be the first to tag this record!

Internet

http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822011000500021

Similar Items