Improvement of a PCR test to diagnose infection by Mansonella ozzardi

Improvement of a PCR test to diagnose infection by Mansonella ozzardi

INTRODUCTION: Mansonelliasis is caused by Mansonella ozzardi. It is widespread in the Amazon region, with a high prevalence. The common exam of thick blood smears stained with Giemsa shows low efficacy levels and has been an obstacle to diagnosing individuals with low blood parasitemia. METHODS: In order to increase diagnosis efficacy, the PCR technique was improved. RESULTS AND CONCLUSIONS: PCR demonstrated the best performance, with sensitivity and negative predictive values (NPV) of 100%, followed by blood filtration through membrane filters, which showed a sensitivity of 88.9% and a NPV of 84.6%, when compared to thick blood smears.

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Main Authors: Vera,Luana Janaína Souza, Basano,Sergio de Almeida, Camargo,Juliana de Souza Almeida Aranha, França,Andonai Krauze de, Ferreira,Ricardo de Godoi Mattos, Casseb,Almeida Andrade, Medeiros,Jansen Fernandes, Fontes,Gilberto, Camargo,Luís Marcelo Aranha
Format: Digital revista
Language:English
Published: Sociedade Brasileira de Medicina Tropical - SBMT 2011
Online Access:http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822011000300023
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spelling oai:scielo:S0037-868220110003000232011-07-11Improvement of a PCR test to diagnose infection by Mansonella ozzardiVera,Luana Janaína SouzaBasano,Sergio de AlmeidaCamargo,Juliana de Souza Almeida AranhaFrança,Andonai Krauze deFerreira,Ricardo de Godoi MattosCasseb,Almeida AndradeMedeiros,Jansen FernandesFontes,GilbertoCamargo,Luís Marcelo Aranha Mansonella ozzardi Diagnosis PCR INTRODUCTION: Mansonelliasis is caused by Mansonella ozzardi. It is widespread in the Amazon region, with a high prevalence. The common exam of thick blood smears stained with Giemsa shows low efficacy levels and has been an obstacle to diagnosing individuals with low blood parasitemia. METHODS: In order to increase diagnosis efficacy, the PCR technique was improved. RESULTS AND CONCLUSIONS: PCR demonstrated the best performance, with sensitivity and negative predictive values (NPV) of 100%, followed by blood filtration through membrane filters, which showed a sensitivity of 88.9% and a NPV of 84.6%, when compared to thick blood smears.info:eu-repo/semantics/openAccessSociedade Brasileira de Medicina Tropical - SBMTRevista da Sociedade Brasileira de Medicina Tropical v.44 n.3 20112011-06-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822011000300023en10.1590/S0037-86822011000300023
institution SCIELO
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country Brasil
countrycode BR
component Revista
access En linea
databasecode rev-scielo-br
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region America del Sur
libraryname SciELO
language English
format Digital
author Vera,Luana Janaína Souza
Basano,Sergio de Almeida
Camargo,Juliana de Souza Almeida Aranha
França,Andonai Krauze de
Ferreira,Ricardo de Godoi Mattos
Casseb,Almeida Andrade
Medeiros,Jansen Fernandes
Fontes,Gilberto
Camargo,Luís Marcelo Aranha
spellingShingle Vera,Luana Janaína Souza
Basano,Sergio de Almeida
Camargo,Juliana de Souza Almeida Aranha
França,Andonai Krauze de
Ferreira,Ricardo de Godoi Mattos
Casseb,Almeida Andrade
Medeiros,Jansen Fernandes
Fontes,Gilberto
Camargo,Luís Marcelo Aranha
Improvement of a PCR test to diagnose infection by Mansonella ozzardi
author_facet Vera,Luana Janaína Souza
Basano,Sergio de Almeida
Camargo,Juliana de Souza Almeida Aranha
França,Andonai Krauze de
Ferreira,Ricardo de Godoi Mattos
Casseb,Almeida Andrade
Medeiros,Jansen Fernandes
Fontes,Gilberto
Camargo,Luís Marcelo Aranha
author_sort Vera,Luana Janaína Souza
title Improvement of a PCR test to diagnose infection by Mansonella ozzardi
title_short Improvement of a PCR test to diagnose infection by Mansonella ozzardi
title_full Improvement of a PCR test to diagnose infection by Mansonella ozzardi
title_fullStr Improvement of a PCR test to diagnose infection by Mansonella ozzardi
title_full_unstemmed Improvement of a PCR test to diagnose infection by Mansonella ozzardi
title_sort improvement of a pcr test to diagnose infection by mansonella ozzardi
description INTRODUCTION: Mansonelliasis is caused by Mansonella ozzardi. It is widespread in the Amazon region, with a high prevalence. The common exam of thick blood smears stained with Giemsa shows low efficacy levels and has been an obstacle to diagnosing individuals with low blood parasitemia. METHODS: In order to increase diagnosis efficacy, the PCR technique was improved. RESULTS AND CONCLUSIONS: PCR demonstrated the best performance, with sensitivity and negative predictive values (NPV) of 100%, followed by blood filtration through membrane filters, which showed a sensitivity of 88.9% and a NPV of 84.6%, when compared to thick blood smears.
publisher Sociedade Brasileira de Medicina Tropical - SBMT
publishDate 2011
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822011000300023
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AT basanosergiodealmeida improvementofapcrtesttodiagnoseinfectionbymansonellaozzardi
AT camargojulianadesouzaalmeidaaranha improvementofapcrtesttodiagnoseinfectionbymansonellaozzardi
AT francaandonaikrauzede improvementofapcrtesttodiagnoseinfectionbymansonellaozzardi
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AT medeirosjansenfernandes improvementofapcrtesttodiagnoseinfectionbymansonellaozzardi
AT fontesgilberto improvementofapcrtesttodiagnoseinfectionbymansonellaozzardi
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