Improvement of a PCR test to diagnose infection by Mansonella ozzardi

Improvement of a PCR test to diagnose infection by Mansonella ozzardi

INTRODUCTION: Mansonelliasis is caused by Mansonella ozzardi. It is widespread in the Amazon region, with a high prevalence. The common exam of thick blood smears stained with Giemsa shows low efficacy levels and has been an obstacle to diagnosing individuals with low blood parasitemia. METHODS: In order to increase diagnosis efficacy, the PCR technique was improved. RESULTS AND CONCLUSIONS: PCR demonstrated the best performance, with sensitivity and negative predictive values (NPV) of 100%, followed by blood filtration through membrane filters, which showed a sensitivity of 88.9% and a NPV of 84.6%, when compared to thick blood smears.

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Bibliographic Details
Main Authors: Vera,Luana Janaína Souza, Basano,Sergio de Almeida, Camargo,Juliana de Souza Almeida Aranha, França,Andonai Krauze de, Ferreira,Ricardo de Godoi Mattos, Casseb,Almeida Andrade, Medeiros,Jansen Fernandes, Fontes,Gilberto, Camargo,Luís Marcelo Aranha
Format: Digital revista
Language:English
Published: Sociedade Brasileira de Medicina Tropical - SBMT 2011
Online Access:http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822011000300023
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http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822011000300023

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