Low sensitivity of polymerase chain reaction for diagnosis of tuberculous meningitis in southeastern Brazil

Low sensitivity of polymerase chain reaction for diagnosis of tuberculous meningitis in southeastern Brazil

Two polymerase chain reaction (PCR) protocols showed low sensitivity (36% and 53% for TB AMPLICOR and MPB64 nested PCR, respectively), when compared with classic microbiological methods (73% and 54% for Ziehl-Neelsen staining and culture, respectively), in the diagnosis of tuberculous meningitis in 91 patients in southeastern Brazil. Only three PCR-positive, microbiologically negative patients were found. Analysis of sequential cerebrospinal fluid samples by nested PCR detected Mycobacterium tuberculosis DNA up to 29 days after the introduction of antituberculosis chemotherapy.

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Bibliographic Details
Main Authors: Brienze,Vânia Maria Sabadoto, Tonon,Ângela Pedroso, Pereira,Fabrício José Tarelho, Liso,Elisabete, Tognola,Waldir Antônio, Santos,Manoel Armando Azevedo dos, Ferreira,Marcelo Urbano
Format: Digital revista
Language:English
Published: Sociedade Brasileira de Medicina Tropical - SBMT 2001
Online Access:http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822001000400015
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