Mixed infection by Histoplasma capsulatum isolates with different mating types in Brazilian AIDS-patients

ABSTRACT Mixed infection by Histoplasma capsulatum isolates with different mating types, in AIDS-patients are described in this study. Morphological, mating type-specific PCR assay and multilocus sequencing type analysis of H. capsulatum isolates recovered from two Brazilian AIDS-patients were performed. Five H. capsulatum isolates were recovered at different times from the two patients. Three isolates were obtained from bone marrow (day 1 – CE0411) and buffy coat cultures (day 1 – CE0311; day 2 – CE0511) of patient 1, and two isolates were isolated from buffy coat cultures (day 3 – CE2813; day 12 – CE2513) of patient 2. The mycelial colonies depicted different textures and pigmentation features. Dimorphic conversion to the yeast-phase in ML-Gema medium was achieved in all isolates. MAT1-1 idiomorph was identified in CE0311, CE0411 and CE2813 isolates; MAT1-2 idiomorph was found in CE0511 and CE2513 isolates. These H. capsulatum isolates were grouped within LAm A clade, highlighting that CE0311 and CE0411 isolates formed a subgroup supported by a high bootstrap value. The CE0511, CE2513, and CE2813 isolates clustered together with a Brazilian H151 isolate. This research reports mixed infections caused by H. capsulatum isolates with different mating types in Brazilian AIDS-patients for the first time in the literature.

Bibliographic Details

Main Authors:	Damasceno,Lisandra Serra, Vite-Garín,Tania, Ramírez,José Antonio, Rodríguez-Arellanes,Gabriela, Almeida,Marcos Abreu de, Muniz,Mauro de Medeiros, Mesquita,Jacó Ricarte Lima de, Leitão,Terezinha do Menino Jesus Silva, Taylor,Maria Lucia, Zancopé-Oliveira,Rosely Maria
Format:	Digital revista
Language:	English
Published:	Instituto de Medicina Tropical de São Paulo 2019
Online Access:	http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652019005000203
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