Molecular typing of dengue virus type 2 in Brazil

Strain typing is a critical tool for molecular epidemiological analysis and can provide important information about the spread of dengue viruses. Here, we performed a molecular characterization of DEN-2 viruses isolated in Brazil during 1990-2000 from geographically and temporally distinct areas in order to investigate the genetic distribution of this serotype circulating in the country. Restriction site-specific polymerase chain reaction (RSS)-PCR presented the same pattern for all 52 Brazilian samples, showing the circulation of just one DEN-2 variant. Phylogenetic analysis using progressive pairwise alignments from 240-nucleotide sequences of the E/NS1 junction in 15 isolates showed that they belong to genotype III (Jamaica genotype).

Bibliographic Details

Main Authors:	Miagostovich,Marize P., Sequeira,Patrícia C., Santos,Flávia B. dos, Maia,Amanda, Nogueira,Rita Maria R., Schatzmayr,Hermann G., Harris,Eva, Riley,Lee W.
Format:	Digital revista
Language:	English
Published:	Instituto de Medicina Tropical de São Paulo 2003
Online Access:	http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652003000100004
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