Polymerase chain reaction (PCR) for the detection of Salmonella in artificially inoculated chicken meat

The aim of this study was to develop a polymerase chain reaction (PCR) protocol for the detection of Salmonella in artificially contaminated chicken meat. Tests were performed with different dilutions of Salmonella Typhimurium or Salmonella Enteritidis cells (10-7, 10-8 or 10-9 CFU/mL) inoculated in chicken meat samples, in order to establish the limits of detection, incubation times (0, 6, 8 and 24 hours of pre-enrichment in PBW 1%) and three DNA extraction protocols (phenol-chloroform, thermal treatment and thermal treatment and Sephaglass). The assay was able to detect until 10-9 CFU/mL of initial dilution of Salmonella cells inoculated in chicken meat, which allows detection of Salmonella within 48 hours, including 24 hours of pre-enrichment and using the phenol-chloroform DNA extraction protocol. As the results are obtained in a shorter time period than that of microbiological culture, this procedure will be useful in the methodology for detection of Salmonella in chicken.

Bibliographic Details

Main Authors:	SANTOS,Luciana Ruschel dos, NASCIMENTO,Vladimir Pinheiro do, OLIVEIRA,Sílvia Dias de, FLORES,Maristela Lovato, PONTES,Alexandre Pontes, RIBEIRO,Aldemir Reginato, SALLE,Carlos Tadeu Pippi, LOPES,Rui Fernando Félix
Format:	Digital revista
Language:	English
Published:	Instituto de Medicina Tropical de São Paulo 2001
Online Access:	http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652001000500002
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