Radiometric studies on the oxidation of (U-14C) L-amino acids by drug-susceptible and drug-resistant mycobacteria

A radiometric assay system has been used to study oxidation patterns of (U-14C) L-amino acids by drug-susceptible and drug-resistant mycobacteria. Drug-susceptible M. tuberculosis (H37Rv TMC 102 and Erdman) along with the drug-resistant organism M. tuberculosis (H37 Rv TMC 303), M. bovis, M. avium, M. intracellulare, M. kansasii and M. chelonei were used. The organisms were inoculated into a sterile reaction system with liquid 7H9 medium and one of the (U-14C) L-amino acids. Each organism displayed a different pattern of amino acid oxidation, but these patterns were not distinctive enough for identification of the organism. Complex amino acids such as proline, phenylalanine and tyrosine were of no use in identification of mycobacteria, since virtually all organisms failed to oxidize them. There was no combination of substrates able to separate susceptible from resistant organisms.

Bibliographic Details

Main Authors:	Camargo,Edwaldo E., Kopajtic,Teresa M., Hopkins,Glinda K., Cannon,Nancy P., Wagner Jr,Henry N.
Format:	Digital revista
Language:	English
Published:	Instituto de Medicina Tropical de São Paulo 1987
Online Access:	http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46651987000500008
Tags:	Add Tag No Tags, Be the first to tag this record!