Immobilization of recognition elements on a self-assembled monolayers bio-platform
Abstract Tailored materials formed by spontaneous two-dimensional arrangement of 3-aminopropyltriethoxysilane self-assembled monolayer on glass (amino-functionalized glass) has been exploited to attach biomolecules in well-organized structures useful in biosensing. Succinimidyl ester of both unpolymerized diacetylene liposome (NHS-DA-liposome) layer and PEGylated biotin (Bt-PEG-NHS) matrix were covalently bonded to the amino-functionalized glass by the NHS linker, and exposed to either Tyrosinase (Ty) or Streptavidin (SAV) solution. The interaction between Ty and polymerized NHS-PDA-liposome transformed the planarity of the PDA backbone, and a blue-to-red transition occurred; Bt-PEG attached to the fluorescent-SAV by bioaffinity. Sensing capability of bioplatform systems was evaluated by Uv-vis spectroscopy or fluorescence microscopy. Biomolecule functionalized SAMs retained the recognition potential of colorimetric Ty-PDA-liposome after biological interaction, and also facilitated the fabrication of a protein-resistant matrix with a particular affinity property. This surface chemistry is accessible to depositing proteins on both SAM-coated glass surface, and tethered to SAM, resulting in optical bioplatform arrays.
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| Format: | Digital revista |
| Language: | English |
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Universidad Nacional de Colombia
2017
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| Online Access: | http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0012-73532017000300263 |
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