Optimal conditions for the kinematic analysis in fresh semen of Brahman bulls with a CASA-Mot system
Introduction. The optimal conditions for semen analysis enable us to standardize the evaluation protocols. Objective. To evaluate the effect of external factors related to the semen analysis on the sperm subpopulations of the Brahman cattle. Materials and Methods. The study was conducted with animals from two cattle farms in Alajuela, Costa Rica, from September to December 2019. Ten Brahman bulls were used that were electroejaculated and the semen was diluted with three commercial diluents: Andromed®, Androstar®, and BTS, at two temperatures (37 and 29 °C). The samples were analyzed using a CASA-Mot ISAS®v1 system and ISAS®D4C counting chambers (10, 16 and 20 µm) and Spermtrack® were used at different analysis times (0, 3, 6 and 12 h). Results. The Spermtrack® presented higher curvilinear velocity but lower linearity compared to the other counting chambers (p<0.05). The samples durability was lower for all the sperm kinematic variables (p<0.05), except for the straightness index (STR). Curvilinear velocity was higher when Andromed® was used, but there was greater progressivity with Androstar® (p<0.05). Five sperm subpopulations were identified from three main components: velocity, progressiveness, and undulation. The distribution of spermatozoa in the subpopulations varied (p<0.05) according to sample durability and counting chamber. Conclusion. The type of diluent, dilution temperature, counting chamber, and time elapsed after initial semen loading conditioned ejaculate kinematic variables. Semen motility and kinematics improved when chamber heights of 20 µm and Androstar® diluent were used. The existence of sperm subpopulations in the ejaculate was affected by the type of extender used, which conditioned the presence of different motility patterns, progressiveness and cell undulation in the different subpopulations within the ejaculate.
Saved in:
| Main Authors: | , , |
|---|---|
| Format: | Digital revista |
| Language: | spa eng |
| Published: |
Universidad de Costa Rica
2021
|
| Online Access: | https://revistas.ucr.ac.cr/index.php/agromeso/article/view/42768 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| id |
oai:portal.ucr.ac.cr:article42768 |
|---|---|
| record_format |
ojs |
| institution |
UCR |
| collection |
OJS |
| country |
Costa Rica |
| countrycode |
CR |
| component |
Revista |
| access |
En linea |
| databasecode |
rev-agromeso |
| tag |
revista |
| region |
America Central |
| libraryname |
Bibioteca de la Facultad de Agronomía |
| language |
spa eng |
| format |
Digital |
| author |
Víquez, Luis Gabriel Barquero, Vinicio Valverde, Anthony |
| spellingShingle |
Víquez, Luis Gabriel Barquero, Vinicio Valverde, Anthony Optimal conditions for the kinematic analysis in fresh semen of Brahman bulls with a CASA-Mot system |
| author_facet |
Víquez, Luis Gabriel Barquero, Vinicio Valverde, Anthony |
| author_sort |
Víquez, Luis Gabriel |
| title |
Optimal conditions for the kinematic analysis in fresh semen of Brahman bulls with a CASA-Mot system |
| title_short |
Optimal conditions for the kinematic analysis in fresh semen of Brahman bulls with a CASA-Mot system |
| title_full |
Optimal conditions for the kinematic analysis in fresh semen of Brahman bulls with a CASA-Mot system |
| title_fullStr |
Optimal conditions for the kinematic analysis in fresh semen of Brahman bulls with a CASA-Mot system |
| title_full_unstemmed |
Optimal conditions for the kinematic analysis in fresh semen of Brahman bulls with a CASA-Mot system |
| title_sort |
optimal conditions for the kinematic analysis in fresh semen of brahman bulls with a casa-mot system |
| description |
Introduction. The optimal conditions for semen analysis enable us to standardize the evaluation protocols. Objective. To evaluate the effect of external factors related to the semen analysis on the sperm subpopulations of the Brahman cattle. Materials and Methods. The study was conducted with animals from two cattle farms in Alajuela, Costa Rica, from September to December 2019. Ten Brahman bulls were used that were electroejaculated and the semen was diluted with three commercial diluents: Andromed®, Androstar®, and BTS, at two temperatures (37 and 29 °C). The samples were analyzed using a CASA-Mot ISAS®v1 system and ISAS®D4C counting chambers (10, 16 and 20 µm) and Spermtrack® were used at different analysis times (0, 3, 6 and 12 h). Results. The Spermtrack® presented higher curvilinear velocity but lower linearity compared to the other counting chambers (p<0.05). The samples durability was lower for all the sperm kinematic variables (p<0.05), except for the straightness index (STR). Curvilinear velocity was higher when Andromed® was used, but there was greater progressivity with Androstar® (p<0.05). Five sperm subpopulations were identified from three main components: velocity, progressiveness, and undulation. The distribution of spermatozoa in the subpopulations varied (p<0.05) according to sample durability and counting chamber. Conclusion. The type of diluent, dilution temperature, counting chamber, and time elapsed after initial semen loading conditioned ejaculate kinematic variables. Semen motility and kinematics improved when chamber heights of 20 µm and Androstar® diluent were used. The existence of sperm subpopulations in the ejaculate was affected by the type of extender used, which conditioned the presence of different motility patterns, progressiveness and cell undulation in the different subpopulations within the ejaculate. |
| publisher |
Universidad de Costa Rica |
| publishDate |
2021 |
| url |
https://revistas.ucr.ac.cr/index.php/agromeso/article/view/42768 |
| work_keys_str_mv |
AT viquezluisgabriel optimalconditionsforthekinematicanalysisinfreshsemenofbrahmanbullswithacasamotsystem AT barquerovinicio optimalconditionsforthekinematicanalysisinfreshsemenofbrahmanbullswithacasamotsystem AT valverdeanthony optimalconditionsforthekinematicanalysisinfreshsemenofbrahmanbullswithacasamotsystem AT viquezluisgabriel condicionesoptimasdeanalisiscineticoensemenfrescodetorosbrahmanconunsistemacasamot AT barquerovinicio condicionesoptimasdeanalisiscineticoensemenfrescodetorosbrahmanconunsistemacasamot AT valverdeanthony condicionesoptimasdeanalisiscineticoensemenfrescodetorosbrahmanconunsistemacasamot |
| _version_ |
1769606459878801408 |
| spelling |
oai:portal.ucr.ac.cr:article427682023-06-16T13:44:06Z Optimal conditions for the kinematic analysis in fresh semen of Brahman bulls with a CASA-Mot system Condiciones óptimas de análisis cinético en semen fresco de toros Brahman con un sistema CASA-Mot Víquez, Luis Gabriel Barquero, Vinicio Valverde, Anthony spermatozoaa livestock counting chamber bull animal reproduction espermatozoide ganado cámara de recuento toro reproducción animal Introduction. The optimal conditions for semen analysis enable us to standardize the evaluation protocols. Objective. To evaluate the effect of external factors related to the semen analysis on the sperm subpopulations of the Brahman cattle. Materials and Methods. The study was conducted with animals from two cattle farms in Alajuela, Costa Rica, from September to December 2019. Ten Brahman bulls were used that were electroejaculated and the semen was diluted with three commercial diluents: Andromed®, Androstar®, and BTS, at two temperatures (37 and 29 °C). The samples were analyzed using a CASA-Mot ISAS®v1 system and ISAS®D4C counting chambers (10, 16 and 20 µm) and Spermtrack® were used at different analysis times (0, 3, 6 and 12 h). Results. The Spermtrack® presented higher curvilinear velocity but lower linearity compared to the other counting chambers (p<0.05). The samples durability was lower for all the sperm kinematic variables (p<0.05), except for the straightness index (STR). Curvilinear velocity was higher when Andromed® was used, but there was greater progressivity with Androstar® (p<0.05). Five sperm subpopulations were identified from three main components: velocity, progressiveness, and undulation. The distribution of spermatozoa in the subpopulations varied (p<0.05) according to sample durability and counting chamber. Conclusion. The type of diluent, dilution temperature, counting chamber, and time elapsed after initial semen loading conditioned ejaculate kinematic variables. Semen motility and kinematics improved when chamber heights of 20 µm and Androstar® diluent were used. The existence of sperm subpopulations in the ejaculate was affected by the type of extender used, which conditioned the presence of different motility patterns, progressiveness and cell undulation in the different subpopulations within the ejaculate. Introducción. Las condiciones óptimas de análisis seminal permiten estandarizar los protocolos de evaluación. Objetivo. Evaluar el efecto de factores externos relacionados con el seminograma sobre las subpoblaciones espermáticas de bovinos de la raza Brahman. Materiales y métodos. El estudio se realizó con animales de dos fincas ganaderas de Alajuela, Costa Rica, de septiembre a diciembre de 2019. Se utilizaron diez toros Brahman que fueron electroeyaculados y el semen se diluyó con tres diluyentes comerciales: Andromed®, Androstar® y BTS, a dos temperaturas (37 y 29 °C). Las muestras se analizaron mediante un sistema CASA-Mot ISAS®v1 y se utilizaron cámaras de recuento ISAS®D4C (10, 16 y 20 μm) y Spermtrack® en diferentes tiempos de análisis (0, 3, 6 y 12 h). Resultados. La Spermtrack® presentó mayor velocidad curvilínea pero menor linealidad respecto de las otras cámaras de recuento (p<0,05). La durabilidad de las muestras fue menor para todas las variables de cinética espermática (p<0,05), excepto para el índice de rectitud (STR). La velocidad curvilínea fue mayor cuando se utilizó Andromed®, pero hubo mayor progresividad con Androstar® (p<0,05). Se identificaron cinco subpoblaciones espermáticas a partir de tres componentes principales: velocidad, progresividad y ondulación. La distribución de espermatozoides en las subpoblaciones varió (p<0,05) según la durabilidad de la muestra y la cámara de recuento. Conclusiones. El tipo de diluyente, la temperatura de dilución, la cámara de recuento y el tiempo transcurrido tras la carga inicial del semen condicionaron las variables cinéticas del eyaculado. La movilidad y cinética del semen mejoró cuando se utilizaron alturas de cámara de 20 µm y diluyente Androstar®. La existencia de subpoblaciones espermáticas en el eyaculado se vio afectada por el tipo de diluyente utilizado, el cual condicionó la presencia de diferentes patrones de movilidad, progresividad y ondulación de las células en las diferentes subpoblaciones dentro del eyaculado. Universidad de Costa Rica 2021-09-01 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Article text/xml application/pdf application/epub+zip text/html audio/mpeg audio/mpeg https://revistas.ucr.ac.cr/index.php/agromeso/article/view/42768 10.15517/am.v32i3.42768 Agronomía Mesoamericana; 2021: Agronomía Mesoamericana: Vol. 32, Issue 3 (September-December); 920-938 Agronomía Mesoamericana; 2021: Agronomía Mesoamericana: Vol. 32, Nº 3 (setiembre-diciembre); 920-938 Agronomía Mesoamericana; 2021: Agronomía Mesoamericana: Vol. 32, Issue 3 (September-December); 920-938 2215-3608 1021-7444 spa eng https://revistas.ucr.ac.cr/index.php/agromeso/article/view/42768/47834 https://revistas.ucr.ac.cr/index.php/agromeso/article/view/42768/47835 https://revistas.ucr.ac.cr/index.php/agromeso/article/view/42768/47836 https://revistas.ucr.ac.cr/index.php/agromeso/article/view/42768/47837 https://revistas.ucr.ac.cr/index.php/agromeso/article/view/42768/47838 https://revistas.ucr.ac.cr/index.php/agromeso/article/view/42768/47839 Copyright (c) 2021 Luis Gabriel Viquez, Vinicio Barquero, Anthony Valverde Abarca |