Caracterización morfológica y molecular de Fusarium oxysporum F. SP. Apii asociado a la marchitez del apio en Costa Rica
Morphological and molecularcharacterization of Fusarium oxysporum f.sp. apii associated to yellow wilt on celery inCosta Rica. Celery is cultivated in provinces ofCosta Rica, such as Cartago, Heredia, Alajuelaand San José. Total production is approximately25 metric tons annually. This crop is affectedby different pathogens but in the last few yearsFusarium oxysporum has caused substantialdamage at the plantation level. In celery, 4pathogenic Fusarium races are known, theyare defined using the tolerance of the celerycultivars to the pathogen. Race 1 affects onlyyellow celery; race 2 affects both yellow andgreen celery; race 3, the least pathogenic, isvirulent only for green celery; and race 4, isthe most aggressive causing losses in green asyellow celery. In Costa Rica only the presenceof Fusarium oxysporum has been reported. Tobetter identify and describe the pathogen, isolateswere obtained from diseased plants that showeda variety of symptoms including: delayed plant growth, dwarfism, yellow and wilting foliage, aswell as blackening and damaged root systems.Koch postulates and a morphological descriptionof the pathogen was done based on differentcharacteristics of spore quantity and the presenceof macro and microconidia on carnation leaf agar(CLA), as well as colony appearance (color andpigmentations), which were observed on potatodextrose agar (PDA). Molecular characterizationwas performed by sequencing the elongationfactor 1-alpha region (EF-1a). Results matchedsome isolates found at the National Center forBiotechnolgy Information (NCBI) related to F.oxysporum f. sp. apii and our results furthersuggested that we are dealing with the presenceof race 3 and/or 4 in Costa Rica.
| Main Authors: | , , , |
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| Format: | Digital revista |
| Language: | spa |
| Published: |
Universidad de Costa Rica
2018
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| Online Access: | https://revistas.ucr.ac.cr/index.php/agrocost/article/view/32199 |
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