Genetic diversity of passion fruits in Guatemala revealed by AFLP markers.
Genetic diversity of passion fruits in Guatemala revealed by AFLP markers. The objectives of this study were to characterize with AFLP nine genotypes of passion fruit collected in Guatemala, to identify clusters and to evaluate the existing genetic diversity. The study was conducted at Instituto de Ciencia y Tecnología Agrícolas (ICTA), during July 2010/May 2011. A preliminary analysis was conducted in which ten selective primer pairs were amplified and 106 polymorphisms were detected. The selective primer pair E+ACG/M+CAG, E+ACA/M+CTA, E+ACT/M+CTG and E+AAC/M+CTT, gave the most information. An average often loci per selective amplification was detected. Similarity analysis revealed no duplicated genotypes. Correspondence and clustering analysis identified two groups. The fi rst one included all accession of P. edulis f. edulis Sims and the second one all accessions of P. edulis f. fl avicarpa Degener.The genetic diversity based on Nei´s index for the collection was 0,3160. Genetic differentiation (Gst) was 0,2542 for theentire collection; 25.42% of the genetic diversity is expressed between groups while the 74,58% is expressed within groups. Those results give evidence of the evolutionary proximity of the purple and yellow types of P. edulis Sims.The genetic flow was high (Nm=1,4670) as expected in anallogamous species in which genetic exchange is facilitated.
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| Format: | Digital revista |
| Language: | spa |
| Published: |
Universidad de Costa Rica
2012
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| Online Access: | https://revistas.ucr.ac.cr/index.php/agromeso/article/view/2136 |
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