Identificación de micorrizas asociadas con el cultivo de Cacao (Theobroma cacao L.) en la finca Experimental Engombe: Identificación de micorrizas asociadas con el cultivo de Cacao (Theobroma cacao L.)
The cocoa plantations in the Dominican Republic are minimally fertilized (5%) and therefore could be considered organic from that point of view. Therefore, identifying the native mycorrhizal population associated with cocoa cultivation in the Engombe agricultural experimental farm, at the Autonomous University of Santo Domingo, Dominican Republic, is an objective of national interest. It was carried out in three phases: 1. Soil and root sampling in cocoa plants with a depth of 0-20 cm for a total of 13 samples, 2. The analysis of the sample in the laboratory that included, the extraction of spores, observation of the infection in the roots, by means of staining and 3. Molecular identification with deoxyribonucleic acid (DNA) and extraction and amplification by means of PCR (Polymerase chain reaction) was applied. In the wet spore extraction, the presence of mycorrhizae dominating the genus of Glomus sp. with 77% of the samples analyzed and 23% by the Acaulospora sp. and Gigaspora with minimal presentation, which shows the presence of native mycorrhizae associated with cocoa cultivation. Later, by means of the PCR test, the genus Glomus was identified in the soil samples. This type of information is of utmost importance for the export of organic cocoa, that is to say reducing the use of chemical fertilizers in the production of this crop and therefore, improving the characteristics of the product in terms of quality and production.
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| Format: | Digital revista |
| Language: | spa |
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Sociedad Dominicana de Investigadores Agropecuarios y Forestales
2021
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| Online Access: | https://www.sodiaf.org.do/apf/index.php/apf/article/view/123 |
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