BEGOMOVIRUSES INFECTING TOMATO IN THE PROVINCE LOS SANTOS, PANAMA: CURRENT SITUATION AND MANAGEMENT
Begomoviruses, transmitted by the whitefly Bemisia tabaci, constitute the group of viruses with the highest incidence and distribution in tomato crop in tropical and subtropical countries. With the objective to determine the incidence of begomoviruses on industrial tomato in Los Santos Province, Panama, a prospection was realized during 2016-2017 period. A total of 70 leaf samples were taken from plants with viral infection symptoms in nine production plots from seven localities, in the main tomato producing zones. Total DNA was extracted from each sample and extracts were analyzed by the polymerase chain reaction technique (PCR), using degenerate primers for begomoviruses. Samples with positive reaction were analyzed with specific primers for the three begomoviruses species infecting tomato in Panama: Potato yellow mosaic Panama virus (PYMPV), Tomato yellow mottle virus (TYMoV) and Tomato leaf curl Sinaloa virus (ToLCSiV). Fifty-eight (58) samples (82,9% from total) were positive to begomoviruses. A 100% of these samples were infected by PYMPV. TYMoV and ToLCSiV were not found in analyzed samples. These results were confirmed by DNA sequencing and sequence analysis. The relation of begomoviruses with the serious damages recently suffered by farmers, due to a depressed tomato production, as well as strategies for begomoviruses-B. tabaci complex management, are discussed in this study.
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| Format: | Digital revista |
| Language: | spa |
| Published: |
Instituto de Innovación Agropecuaria de Panamá
2018
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| Online Access: | http://www.revistacienciaagropecuaria.ac.pa/index.php/ciencia-agropecuaria/article/view/160 |
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