In vitro GAS PRODUCTION AND FERMENTATIVE CHARACTERISTICS OF RUMINAL CELLULOLYTIC BACTERIAL CONSORTIA OF WATER BUFFALO (Bubalus bubalis) AND SUIZ-BU COW.
The cellulolytic bacterial consortia (CBC) serve as additives in the feed of ruminants to improve the degradation of cellulose and hemicellulose. The objective of the present study was to determine the in vitro production of gas and the fermentative characteristics of CBC of water buffalo and Suiz-bu cow in coculture with ruminal bacteria of Suiz-bu cow. The ruminal fluid of a water buffalo and of a Suiz-bu cow were inoculated in media of crystalline cellulose Whatman® paper to obtain CBC of the buffalo cow (CBCbuf) and of the Suiz-bu cow (CBCcebu). The experimental design was completely randomized and the treatments were the inocula: total ruminal bacteria (TRB), CBCceb, CBCbuf, cocultureceb (TRB and CBCceb) and coculturebuf (TRB and CBCbuf). The biodigesters contained 0.5 g of cobra grass, 45 mL of culture medium and 5 mL of inoculum. Gas production was measured at 3, 6, 9, 12, 24, 48 and 72 h, and that of methane (CH4 ) at 24, 48 and 72 h. The variables evaluated were pH, ammonia nitrogen (N-NH3 ), dry matter degradation (DEGDM) and NDF degradation (DEGNDF), and the bacteria were counted. CBCbuf and CBCbov produced a higher amount (p£0.05) of gas and CH4 accumulated at 72 h, and increased (p£0.05) DEGDM and DEGNDF, but without differences between them (p>0.05). TRB did not present differences (p>0.05) with the cocultureceb in the DEGDM and with coculturebuf in the DEGNDF. The inocula with CBC did not present differences (p>0.05) in pH. Furthermore, the bacteria count and N-NH3 were similar in the media evaluated with different source of inoculum (p>0.05). The cellulolytic bacterial consortia increased production of gas, CH4 , DM degradation and NDF degradation when they were added to the ruminal bacteria under in vitro conditions.
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Colegio de Postgraduados
2019
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Torres-Salado, Nicolás Sánchez-Santillán, Paulino Rojas-García, R. Adelaido Almaraz-Buendía, Isaac Herrera-Pérez, Jerónimo Reyes-Vázquez, Iván F. Jesús Mayren-Mendoza |
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Torres-Salado, Nicolás Sánchez-Santillán, Paulino Rojas-García, R. Adelaido Almaraz-Buendía, Isaac Herrera-Pérez, Jerónimo Reyes-Vázquez, Iván F. Jesús Mayren-Mendoza In vitro GAS PRODUCTION AND FERMENTATIVE CHARACTERISTICS OF RUMINAL CELLULOLYTIC BACTERIAL CONSORTIA OF WATER BUFFALO (Bubalus bubalis) AND SUIZ-BU COW. |
| author_facet |
Torres-Salado, Nicolás Sánchez-Santillán, Paulino Rojas-García, R. Adelaido Almaraz-Buendía, Isaac Herrera-Pérez, Jerónimo Reyes-Vázquez, Iván F. Jesús Mayren-Mendoza |
| author_sort |
Torres-Salado, Nicolás |
| title |
In vitro GAS PRODUCTION AND FERMENTATIVE CHARACTERISTICS OF RUMINAL CELLULOLYTIC BACTERIAL CONSORTIA OF WATER BUFFALO (Bubalus bubalis) AND SUIZ-BU
COW. |
| title_short |
In vitro GAS PRODUCTION AND FERMENTATIVE CHARACTERISTICS OF RUMINAL CELLULOLYTIC BACTERIAL CONSORTIA OF WATER BUFFALO (Bubalus bubalis) AND SUIZ-BU
COW. |
| title_full |
In vitro GAS PRODUCTION AND FERMENTATIVE CHARACTERISTICS OF RUMINAL CELLULOLYTIC BACTERIAL CONSORTIA OF WATER BUFFALO (Bubalus bubalis) AND SUIZ-BU
COW. |
| title_fullStr |
In vitro GAS PRODUCTION AND FERMENTATIVE CHARACTERISTICS OF RUMINAL CELLULOLYTIC BACTERIAL CONSORTIA OF WATER BUFFALO (Bubalus bubalis) AND SUIZ-BU
COW. |
| title_full_unstemmed |
In vitro GAS PRODUCTION AND FERMENTATIVE CHARACTERISTICS OF RUMINAL CELLULOLYTIC BACTERIAL CONSORTIA OF WATER BUFFALO (Bubalus bubalis) AND SUIZ-BU
COW. |
| title_sort |
in vitro gas production and fermentative characteristics of ruminal cellulolytic bacterial consortia of water buffalo (bubalus bubalis) and suiz-bu
cow. |
| description |
The cellulolytic bacterial consortia (CBC) serve as additives in the feed of ruminants to improve the degradation of cellulose and hemicellulose. The objective of the
present study was to determine the in vitro production of gas and the fermentative characteristics of CBC of water buffalo and Suiz-bu cow in coculture with ruminal bacteria of Suiz-bu cow.
The ruminal fluid of a water buffalo and of a Suiz-bu cow were inoculated in media of crystalline cellulose Whatman® paper to obtain CBC of the buffalo cow (CBCbuf) and of the Suiz-bu cow
(CBCcebu). The experimental design was completely randomized and the treatments were the inocula: total ruminal bacteria (TRB), CBCceb, CBCbuf, cocultureceb (TRB and CBCceb) and coculturebuf
(TRB and CBCbuf). The biodigesters contained 0.5 g of cobra grass, 45 mL of culture medium and 5 mL of inoculum. Gas production was measured at 3, 6, 9, 12, 24, 48 and 72 h, and that of
methane (CH4 ) at 24, 48 and 72 h. The variables evaluated were pH, ammonia nitrogen (N-NH3 ), dry matter degradation (DEGDM) and NDF degradation (DEGNDF), and the bacteria were counted.
CBCbuf and CBCbov produced a higher amount (p£0.05) of gas and CH4 accumulated at 72 h, and increased (p£0.05) DEGDM and DEGNDF, but without differences between them (p>0.05). TRB did not
present differences (p>0.05) with the cocultureceb in the DEGDM and with coculturebuf in the DEGNDF. The inocula with CBC did not present differences (p>0.05) in pH. Furthermore, the
bacteria count and N-NH3 were similar in the media evaluated with different source of inoculum (p>0.05). The cellulolytic bacterial consortia increased production of gas, CH4 , DM
degradation and NDF degradation when they were added to the ruminal bacteria under in vitro conditions. |
| publisher |
Colegio de Postgraduados |
| publishDate |
2019 |
| url |
https://www.agrociencia-colpos.org/index.php/agrociencia/article/view/1775 |
| work_keys_str_mv |
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| _version_ |
1807208711490371584 |
| spelling |
oai:ojs.pkp.sfu.ca:article17752020-05-14T08:09:17Z In vitro GAS PRODUCTION AND FERMENTATIVE CHARACTERISTICS OF RUMINAL CELLULOLYTIC BACTERIAL CONSORTIA OF WATER BUFFALO (Bubalus bubalis) AND SUIZ-BU COW. PRODUCCIÓN DE GAS in vitro Y CARACTERÍSTICAS FERMENTATIVAS DE CONSORCIOS BACTERIANOS CELULOLÍTICOS RUMINALES DE BÚFALA DE AGUA (Bubalus bubalis) Y VACA SUIZ-BU Torres-Salado, Nicolás Sánchez-Santillán, Paulino Rojas-García, R. Adelaido Almaraz-Buendía, Isaac Herrera-Pérez, Jerónimo Reyes-Vázquez, Iván F. Jesús Mayren-Mendoza forage cocultures ruminal bacteria in vitro rumiants forrajes cocultivos bacterias ruminales in vitro rumiantes The cellulolytic bacterial consortia (CBC) serve as additives in the feed of ruminants to improve the degradation of cellulose and hemicellulose. The objective of the present study was to determine the in vitro production of gas and the fermentative characteristics of CBC of water buffalo and Suiz-bu cow in coculture with ruminal bacteria of Suiz-bu cow. The ruminal fluid of a water buffalo and of a Suiz-bu cow were inoculated in media of crystalline cellulose Whatman® paper to obtain CBC of the buffalo cow (CBCbuf) and of the Suiz-bu cow (CBCcebu). The experimental design was completely randomized and the treatments were the inocula: total ruminal bacteria (TRB), CBCceb, CBCbuf, cocultureceb (TRB and CBCceb) and coculturebuf (TRB and CBCbuf). The biodigesters contained 0.5 g of cobra grass, 45 mL of culture medium and 5 mL of inoculum. Gas production was measured at 3, 6, 9, 12, 24, 48 and 72 h, and that of methane (CH4 ) at 24, 48 and 72 h. The variables evaluated were pH, ammonia nitrogen (N-NH3 ), dry matter degradation (DEGDM) and NDF degradation (DEGNDF), and the bacteria were counted. CBCbuf and CBCbov produced a higher amount (p£0.05) of gas and CH4 accumulated at 72 h, and increased (p£0.05) DEGDM and DEGNDF, but without differences between them (p>0.05). TRB did not present differences (p>0.05) with the cocultureceb in the DEGDM and with coculturebuf in the DEGNDF. The inocula with CBC did not present differences (p>0.05) in pH. Furthermore, the bacteria count and N-NH3 were similar in the media evaluated with different source of inoculum (p>0.05). The cellulolytic bacterial consortia increased production of gas, CH4 , DM degradation and NDF degradation when they were added to the ruminal bacteria under in vitro conditions. Los consorcios bacterianos celulolíticos (CBC) sirven como aditivos en la alimentación de rumiantes para mejorar la degradación de celulosa y hemicelulosa. El objetivo de este estudio fue determinar la producción de gas in vitro y las características fermentativas de CBC de búfala de agua y vaca Suiz-bu en cocultivo con bacterias ruminales de vaca Suiz-bu. El fluido ruminal de una búfala de agua y de una vaca Suizbu se inocularon en medios de papel Whatman® y celulosa cristalina para obtener CBC de la búfala (CBCbuf) y de la vaca Suiz-bu (CBCceb). El diseño experimental fue completamente al azar y los tratamientos fueron los inóculos: bacterias ruminales totales (BRT), CBCceb, CBCbuf, cocultivoceb (BRT y CBCceb) y cocultivobuf (BRT y CBCbuf). Los biodigestores usados contenían 0.5 g de pasto cobra, 45 mL de medio de cultivo y 5 mL de inóculo. La producción de gas se midió a las 3, 6, 9, 12, 24, 48 y 72 h y la de metano (CH4 ) a las 24, 48 y 72 h. Las variables evaluadas fueron pH, nitrógeno amoniacal (N-NH3 ), degradación de MS (DEGMS) y degradación de FDN (DEGFDN) y las bacterias se contabilizaron. CBCbuf y CBCbov produjeron mayor (p£0.05) cantidad de gas y CH4 acumulado a las 72 h, incrementaron (p£0.05) DEGMS y DEGFDN, pero sin diferencias entre ellos (p>0.05). BRT no presentó diferencias (p>0.05) con el cocultivoceb en la DEGMS y con cocultivobuf en la DEGFDN. Los inóculos con CBC no presentaron diferencias (p>0.05) en pH; además, el conteo de bacterias y N-NH3 fue similar en los medios evaluados con diferente fuente de inóculo (p>0.05). Los consorcios bacterianos celulolíticos incrementan la producción de gas, CH4 , degradación de MS y de FDN cuando se adicionan a las bacterias ruminales en condiciones in vitro. Colegio de Postgraduados 2019-03-31 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://www.agrociencia-colpos.org/index.php/agrociencia/article/view/1775 Agrociencia; Vol. 53 No. 2 (2019): 16 de febrero - 31 de marzo 2019.; 145-159 Agrociencia; Vol. 53 Núm. 2 (2019): 16 de febrero - 31 de marzo 2019.; 145-159 2521-9766 1405-3195 spa https://www.agrociencia-colpos.org/index.php/agrociencia/article/view/1775/1772 |