OSMOPRIMING OF BEAN SEEDS: SET UP AND CHARACTERIZATION
Osmopriming is a technique which has allowed to reinvigorate seeds of several species. The conditions for osmopriming bean (Phaseolus vulgaris L.) seeds were established. By varyng solutions of PEG-8000 it was found that an osmotic potential of -0.8 MPa inhibited germination. Once the osmotic agent was removed, osmoprimed seeds showed an improvement in their rate of germination. Furthermore, deteriorated seeds showed a recovered vigour when these were osmoprimed. It was found that embryo axes from treated seeds had a limited capacity to incorporate (3Hmethyl) thymidine to DNA under osmopriming conditions; however, the rate of precursor incorporation to DNA notably increased when these seed axes were put to germinate. DNA polymerase activity also increased during germination after osmopriming, showing a cyclic behaviour that was not observed during germination of control seed.
| Main Authors: | , , , |
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| Format: | Digital revista |
| Language: | spa |
| Published: |
Colegio de Postgraduados
1997
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| Online Access: | https://www.agrociencia-colpos.org/index.php/agrociencia/article/view/1494 |
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