Acclimatization and Hardening of Oil Palm Materials Obtained by Techniques of Plant Tissue Culture

The conditioning of plantlets obtaining by plant tissue culture techniques is a transitional stage between the laboratory and the field, which objective is to keep the seedlings from an in vitro to ex vitro conditions. During this stage exists a gradual return to autotrophic functioning and a recovery of normal physiological and morphological characteristics. Cenipalma has standardized a methodology of propagation oil palm by plant tissues culture, which opens the possibility of delivering oil palm clonal materials to the Colombian palm cultivation. However, one of the bottlenecks of the cloning process might be the acclimatization and hardening stages that could be developed for palm growers to have clonal plants in their plantations. Therefore, in the Experimental Field Palmar de la Vizcaina - CEPV (Barrancabermeja) a methodology was adapted for acclimatization of clones as final stage of plant tissue culture propagation, with the future aim of training palm growers interested in planting clones, so they can carry out this acclimatization on their plantations. In this experiment we managed to obtain percentages of acclimatization over 85% in the seedlings classified as type 3 laboratory (good looking, good number of roots and leaves, and larger than 10 cm) and type 2 (intermediate appearance seedlings). Likewise, similar values were obtained for some type seedlings showed abnormalities AC, Curved Double, Double and Triple. Also, the behavior in pre-nursery demonstrated the importance of the quality of the root for the survival of the plant in this stage.

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Bibliographic Details
Main Authors: Sánchez Rodríguez, Luz Ángela, Saavedra Hortúa, Daniel, Romero, Hernán Mauricio
Format: Digital revista
Language:spa
Published: Fedepalma 2012
Online Access:https://publicaciones.fedepalma.org/index.php/palmas/article/view/10739
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