Induction of the acrosomal reaction in mouse spermatozoa by the solution of pellucid zone from alpaca (Lama pacos L.)
Acrosin is one of the principal components in the acrosome, has trypsin-like activity and is secreted after the Acrosome Reaction (RA). The aim of this study was to study the induction of the acrosomal reaction in mouse sperms with homologous- Pellucid Zone and heterologous- Pellucid Zone. Mouse sperms were capacitated by two hours in IVF medium, supplemented with albumin, heparin and synthetic serum to 37 °C and 5% CO2 , and before they were incubated in absence of ZP and in present of solubilized ZP from mouse (0,78 mg/ml) and alpaca (0,78 mg/ml y 2,35 mg/ml). The RA was evaluated through immunocytochemistry with monoclonal antibodies anti-human acrosin C5F10 within intervals of 1 hour during four hours. The results show a signifi cant increase (p<0,05) in acrosome reaction induced with homologous-ZP and heterologous-ZP.
| Main Authors: | , , |
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| Format: | Digital revista |
| Language: | spa |
| Published: |
Universidad Nacional Mayor de San Marcos, Facultad de Ciencias Biológicas
2006
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| Online Access: | https://revistasinvestigacion.unmsm.edu.pe/index.php/rpb/article/view/2351 |
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